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Titolo:
An electrophysiological study on the effects of Pa-1G (a phospholipase A(2)) from the venom of king brown snake, Pseudechis australis, on neuromuscular function
Autore:
Fatehi, M; Rowan, EG; Harvey, AL;
Indirizzi:
Khorasan Univ Med Sci, Fac Med, Dept Pharmacol, Mashhad, Iran Khorasan Univ Med Sci Mashhad Iran c Med, Dept Pharmacol, Mashhad, Iran Univ Strathclyde, Dept Physiol & Pharmacol, Glasgow G4 0NR, Lanark, Scotland Univ Strathclyde Glasgow Lanark Scotland G4 0NR G4 0NR, Lanark, Scotland
Titolo Testata:
TOXICON
fascicolo: 1, volume: 40, anno: 2002,
pagine: 69 - 75
SICI:
0041-0101(200201)40:1<69:AESOTE>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOTOR-NERVE TERMINALS; MOUSE; CURRENTS; TOXINS; POTENTIALS; FROG;
Keywords:
phospholipase A(2) toxin; neuromuscular transmission; nerve terminal action potential; sodium current; potassium currents; patch clamp;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Fatehi, M Khorasan Univ Med Sci, Fac Med, Dept Pharmacol, POB 91775-1843, Mashhad, Iran Khorasan Univ Med Sci POB 91775-1843 Mashhad Iran ashhad, Iran
Citazione:
M. Fatehi et al., "An electrophysiological study on the effects of Pa-1G (a phospholipase A(2)) from the venom of king brown snake, Pseudechis australis, on neuromuscular function", TOXICON, 40(1), 2002, pp. 69-75

Abstract

The effects of Pa-1G, a phospholipase A(2) (PLA(2)) from the venom of the Australian king brown snake (Pseudechis australis) were determined on the release of acetylcholine, muscle resting membrane potential and motor nerve terminal action potential at mouse neuromuscular junction. Intracellular recording from endplate regions of mouse triangularis sterni nerve-muscle preparations revealed that Pa-1G (800 nM) significantly reduced the amplitude of endplate potentials within 10 min exposure. The quantal content of endplate potentials was decreased to 58 +/- 6% of control after 30 min exposure to 800 nM Pa-1G. The toxin also caused a partial depolarisation of mouse muscle fibres within 60 min exposure. Extracellular recording of action potentials at motor nerve terminals showed that Pa-1G reduced the waveforms associated with both sodium and potassium conductances. To investigate whether this was a direct or indirect effect of the toxin on these ionic currents, whole cell patch clamp experiments were performed using human neuroblastoma(SK-N-SH) cells and B82 mouse fibroblasts stably transfected with rKvI.2. Patch clamp recording experiments confirmed that potassium currents sensitive to alpha -dendrotoxin recorded from B82 cells and sodium currents in SK-N-SH cells were not affected by the toxin. Since neither facilitation of acetylcholine release at mouse neuromuscular junction nor depression of potassium currents in B82 cells has been observed, the apparent blockade of potassium currents at mouse motor nerve endings induced by the toxin is unlikely to be due to a selective block of potassium channels. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 17:49:29