Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Atresia revisited: two basic patterns of atresia of bovine antral follicles
Autore:
Irving-Rodgers, HF; van Wezel, IL; Mussard, ML; Kinder, JE; Rodgers, RJ;
Indirizzi:
Flinders Univ S Australia, Dept Med, Bedford Pk, SA 5042, Australia Flinders Univ S Australia Bedford Pk SA Australia 5042 SA 5042, Australia Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA Univ Nebraska LincolnNE USA 68583 , Dept Anim Sci, Lincoln, NE 68583 USA
Titolo Testata:
REPRODUCTION
fascicolo: 5, volume: 122, anno: 2001,
pagine: 761 - 775
SICI:
1470-1626(200111)122:5<761:ARTBPO>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; FOLLICULAR ATRESIA; MEMBRANA GRANULOSA; OVARIAN FOLLICLES; APOPTOSIS; IDENTIFICATION; IMMUNOLOCALIZATION; LOCALIZATION; MACROPHAGES; NIDOGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Irving-Rodgers, HF Flinders Univ S Australia, Dept Med, Bedford Pk, SA 5042, Australia Flinders Univ S Australia Bedford Pk SA Australia 5042
Citazione:
H.F. Irving-Rodgers et al., "Atresia revisited: two basic patterns of atresia of bovine antral follicles", REPRODUCT, 122(5), 2001, pp. 761-775

Abstract

Our observations of bovine follicles indicated that the original histological classifications of atresia were inaccurate. A detailed histological, ultrastructural and immunohistochemical study of antral follicles from bovineovaries collected from an abattoir and from animals whose large follicles had been monitored by ultrasonography was conducted to investigate this further. Nidogen and CD68 were immunolocalized to observe the follicular basallamina and macrophages, respectively. In randomly collected ovaries, approximately one quarter of all antral follicles were undergoing antral atresia, as designated in this study. Antral atresia was characterized by early destruction of the layers of the membrana granulosa closest to the antrum, whereas the most basal cells remained intact. Numerous pyknotic nuclei were observed in the most antral layers and in the antrum close to the membrana granulosa. This is the classic description of atretic follicles and was observed at all sizes of follicle development and almost universally in large follicles (> 5 mm in diameter), including dominant follicles. Basal atretic follicles, as designated in this study, were almost as prevalent as the antral atretic follicles, and were characterized by initial destruction of themost basal layer of granulosa cells, whereas the cells in the most antral layers remained associated with each other and were predominantly healthy. Pyknotic nuclei and the nuclei of dying basal cells budded into apoptotic bodies were observed rarely. The basal lamina of basal atretic follicles wasoften breached by macrophages, which were phagocytosing dying basal granulosa cells. The theca was characterized by an increased deposition of collagen, and the cells were orientated randomly, rather than lying parallel to the membrana granulosa as in healthy follicles. Basal atresia occurred in small (< 5 mm in diameter) follicles only. Importantly, these basal atretic follicles were originally identified incorrectly in the literature. Thus, onthe basis of the results of this study and another on the expression of steroidogenic enzymes in atretic follicles, it is suggested that the standardbiochemical methods for measuring steroid hormone concentrations in follicular fluids to assess atresia should be re-evaluated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:24:28