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Titolo:
Autoradiographic determination of angiotensin II receptors in prepubertal and postpubertal pig ovarian tissue
Autore:
Shuttleworth, G; Hunter, MG; Pipkin, FB;
Indirizzi:
Univ Nottingham, Queens Med Ctr, Sch Human Dev, Div Obstet & Gynecol, Nottingham NG7 2UH, England Univ Nottingham Nottingham England NG7 2UH , Nottingham NG7 2UH, England Univ Nottingham, Sch Biosci, Div Anim Physiol, Sutton LE12 5RD, Leics, England Univ Nottingham Sutton Leics England LE12 5RD on LE12 5RD, Leics, England
Titolo Testata:
REPRODUCTION
fascicolo: 5, volume: 122, anno: 2001,
pagine: 701 - 710
SICI:
1470-1626(200111)122:5<701:ADOAIR>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIRECT OBLIGATE ROLE; RAT OVARY; GRANULOSA-CELLS; QUANTITATIVE AUTORADIOGRAPHY; CONCISE COMMUNICATION; FOLLICULAR SIZE; ESTROUS-CYCLE; BINDING-SITES; THECAL CELLS; LOCALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Shuttleworth, G AgRes, Reprod Technol, POB 40063,Upper Hutt, Wellington, New Zealand AgRes POB 40063,Upper Hutt Wellington New Zealand ealand
Citazione:
G. Shuttleworth et al., "Autoradiographic determination of angiotensin II receptors in prepubertal and postpubertal pig ovarian tissue", REPRODUCT, 122(5), 2001, pp. 701-710

Abstract

The aim of this study was to determine whether specific receptors for angiotensin II are present in prepubertal and postpubertal pig ovaries using anautoradiographic technique and computerized densitometry. Frozen sections were cut from prepared samples, and I-125-labelled angiotensin II and the angiotensin II receptor subtype-specific nonpeptide antagonists for receptorsubtype 1, AT(1) (GRI17289) and subtype 2, AT(2) (PD123319) were used. In both pre- and postpubertal pig ovarian tissue, specific receptors for angiotensin II were demonstrated. These receptors had a density of 2487.6 (range: 267.5-5177.6, n = 4) and 3703.8 (range: 1819.9-5207.8, n = 4) fmol per mm(2), respectively, and dissociation constants of 130.0 and 26.3 nmol l(-1),respectively (prepubertal ovarian range: 106.0-165.4 nmol l(-1); postpubertal ovarian range: 26.1-100.3 nmol l(-1); P < 0.05, Mann-Whitney U test). AT(1) receptors with a Ki for I-125-labelled angiotensin If of 346.9 nmol l(-1) in the prepubertal and 268.1 nmol l(-1) in the postpubertal ovary were located predominantly in follicle wall tissue. Competitive inhibition studies using both angiotensin II antagonists resulted in a decrease in Ki with prepubertal tissue (283.7 nmol l(-1)) and an increase in postpubertal tissue (293.9 nmol l(-1)). Immunocytochemistry using sections from paraffin wax-embedded prepubertal (n = 4) and postpubertal (n = 4) pig ovaries confirmedthe presence of AT, receptors on the granulosa cell layer, but not the thecal cell layer, of antral follicles in both pre- and postpubertal pig ovarian tissue, and AT(2) receptors within the granulosa cell layer of prepubertal pig ovarian antral follicles. In summary, these results indicate that angiotensin II receptors are of higher affinity in postpubertal tissue than they are in prepubertal tissue, and indicate an active renin-angiotensin system within the pig ovary.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 23:25:54