Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Parallel extraction columns and parallel analytical columns coupled with liquid chromatography/tandem mass spectrometry for on-line simultaneous quantification of a drug candidate and its six metabolites in dog plasma
Autore:
Xia, YQ; Hop, CECA; Liu, DQ; Vincent, SH; Chiu, SHL;
Indirizzi:
Merck & Co Inc, Merck Res Labs, Dept Drug Metab, Rahway, NJ 07065 USA Merck & Co Inc Rahway NJ USA 07065 Dept Drug Metab, Rahway, NJ 07065 USA
Titolo Testata:
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
fascicolo: 22, volume: 15, anno: 2001,
pagine: 2135 - 2144
SICI:
0951-4198(2001)15:22<2135:PECAPA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-THROUGHPUT; DIRECT-INJECTION; QUANTITATIVE BIOANALYSIS; BIOLOGICAL SAMPLES; LC/MS/MS; SYSTEM; NEED;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Xia, YQ Merck & Co Inc, Merck Res Labs, Dept Drug Metab, RY80L-109, Rahway, NJ 07065 USA Merck & Co Inc RY80L-109 Rahway NJ USA 07065 Rahway, NJ 07065 USA
Citazione:
Y.Q. Xia et al., "Parallel extraction columns and parallel analytical columns coupled with liquid chromatography/tandem mass spectrometry for on-line simultaneous quantification of a drug candidate and its six metabolites in dog plasma", RAP C MASS, 15(22), 2001, pp. 2135-2144

Abstract

A method with parallel extraction columns and parallel analytical columns (PEC-PAC) for on-line high-flow liquid chromatography/tandem mass spectrometry (LC/MS/MS) was developed and validated for simultaneous quantification of a drug candidate and its six metabolites in dog plasma. Two on-line extraction columns were used in parallel for sample extraction and two analytical columns were used in parallel for separation and analysis. The plasma samples, after addition of an internal standard solution, were directly injected onto the PEC-PAC system for purification and analysis. This method allowed the use of one of the extraction columns for analyte purification whilethe other was being equilibrated. Similarly, one of the analytical columnswas employed to separate the analytes while the other was undergoing equilibration. Therefore, the time needed for reconditioning both extraction andanalytical columns was not added to the total analysis time, which resulted in a shorter run time and higher throughput. Moreover, the on-line columnextraction LC/MS/MS method made it possible to extract and analyze all seven analytes simultaneously with good precision and accuracy despite their chemical class diversity that included primary, secondary and tertiary amines, an alcohol, an aldehyde and a carboxylic acid. The method was validated with the standard curve ranging from 5.00 to 5000 ng/mL. The intra- and inter-day precision was no more than 8% CV and the assay accuracy was between 95 and 107%. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 22:21:13