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Titolo:
Identification, cloning and characterisation of a novel copper-metallothionein in Tetrahymena pigmentosa. sequencing of cDNA and expression
Autore:
Santovito, G; Irato, P; Palermo, S; Boldrin, F; Hunziker, P; Piccinni, E;
Indirizzi:
Univ Padua, Dept Biol, I-35131 Padua, Italy Univ Padua Padua Italy I-35131 iv Padua, Dept Biol, I-35131 Padua, Italy Univ Zurich, Inst Biochem, CH-8057 Zurich, Switzerland Univ Zurich Zurich Switzerland CH-8057 chem, CH-8057 Zurich, Switzerland
Titolo Testata:
PROTIST
fascicolo: 3, volume: 152, anno: 2001,
pagine: 219 - 229
SICI:
1434-4610(200109)152:3<219:ICACOA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRAB CALLINECTES-SAPIDUS; MESSENGER-RNA; CADMIUM-METALLOTHIONEIN; PYRIFORMIS; INDUCTION; GENE; DEGRADATION; THERMOPHILA; REGION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Piccinni, E Univ Padua, Dept Biol, Via U Bassi 58-B, I-35131 Padua, Italy Univ Padua Via U Bassi 58-B Padua Italy I-35131 Padua, Italy
Citazione:
G. Santovito et al., "Identification, cloning and characterisation of a novel copper-metallothionein in Tetrahymena pigmentosa. sequencing of cDNA and expression", PROTIST, 152(3), 2001, pp. 219-229

Abstract

The protist Tetrahymena pigmentosa accumulates large amounts of metal ions, particularly cadmium and copper. This capability is linked to the induction of metallothioneins (MTs), cysteine-rich metal-binding proteins found inprotists, plants and animals. The present study focuses on a novel inducible MT isoform isolated from Tetrahymena after exposure to a non-toxic dose of copper. The cDNA sequence was determined utilising the partial peptide sequence of purified protein. The Cu-MT cDNA encodes 96 amino acids containing 28 cysteine residues (29%) arranged in motifs characteristic of the metal-binding regions of vertebrate and invertebrate MTs. Both the amino acid and nucleotide sequences differ, not only from other animal MTs, but also from the previously characterised Tetrahymena Cd-MT. Both MTs contain the structural pattern GTXXXCKCXXCKC, which may be proposed as a conservative sequence of Tetrahymena MTs. Cu-dependent regulation of MT expression was also investigated by measuring MT-mRNA and MT levels. MT synthesis occurs very quickly and MT contents increase with Cu accumulation. The induction of Cu-MT mRNA is very rapid, with no observable lag period, and is characterised by transient fluctuation, similar to that described for Cd-MT mRNA. The datareported here indicate that, also in the unicellular organism Tetrahymena,two very different MT isoforms, which perform different biological functions, are expressed according to the inducing metal, Cu or Cd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 06:40:46