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Titolo:
Prostaglandin E-2 modulation of vascular endothelial growth factor production in murine macrophages
Autore:
Mukutmoni, M; Hubbard, NE; Erickson, KL;
Indirizzi:
Univ Calif Davis, Sch Med, Dept Cell Biol & Human Anat, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 iol & Human Anat, Davis, CA 95616 USA
Titolo Testata:
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS
fascicolo: 3, volume: 65, anno: 2001,
pagine: 123 - 131
SICI:
0952-3278(200109)65:3<123:PEMOVE>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; POLYUNSATURATED FATTY-ACIDS; NITRIC-OXIDE SYNTHASE; FISH-OIL; PERITONEAL-MACROPHAGES; SYNOVIAL FIBROBLASTS; PERMEABILITY FACTOR; FACTOR EXPRESSION; TUMOR-CELLS; DIETARY ENRICHMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Erickson, KL Univ Calif Davis, Sch Med, Dept Cell Biol & Human Anat, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 nat, Davis, CA 95616 USA
Citazione:
M. Mukutmoni et al., "Prostaglandin E-2 modulation of vascular endothelial growth factor production in murine macrophages", PROS LEUK E, 65(3), 2001, pp. 123-131

Abstract

We have previously shown that dietary (n-3) fatty acids decrease mammary tumor vascularization and PGE(2) production. One possible mechanism may be the modulation of vascular endothelial growth factor (VEGF) production by PGE(2). Macrophages are major producers of VEGF, and thus we assessed the role of PGE(2) in vitro and in vivo on their VEGF production. When added to macrophages, pharmacological (10(-7) M) but not physiological (10(-9) to 10(-11) M) concentrations of PGE(2) increased VEGF mRNA and protein levels. That increased expression was relatively rapid and sustained up to 8 hrs, but declined by 24 hrs. Similarly, dibutryl cAMP increased production of VEGF protein which was completely inhibited by H89. Addition of cAMP-elevating agents further potentiated the production of VEGF by PGE(2). Next, (n-3) and (n-6) fatty acids were added to macrophages in vitro or provided in the diet. Macrophages of mice fed safflower oil (n-6) had 2- to 4-fold greater copy number of VEGF transcripts after lipopolysaccarhide (LPS) stimulation compared to fish oil (n-3). A decreasing trend was seen in LPS-induced VEGF secretion from macrophages in vitro after docosahexaenoic acid or eicosapentaenoic acid incubation compared to arachidonic acid. While pharmacological concentrations of PGE(2) modulate VEGF expression, physiological alterationsdid not alter VEGF protein production by macrophages. (C) 2001 Harcourt Publishers Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 09:35:30