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Titolo:
In vivo target promoter-binding activities of a xenobiotic stress-activated TGA factor
Autore:
Johnson, C; Boden, E; Desai, M; Pascuzzi, P; Arias, J;
Indirizzi:
Univ Maryland, Inst Biotechnol, Agr Biotechnol Ctr, College Pk, MD 20742 USA Univ Maryland College Pk MD USA 20742 chnol Ctr, College Pk, MD 20742 USA Univ Maryland, Program Mol & Cell Biol, College Pk, MD 20742 USA Univ Maryland College Pk MD USA 20742 Cell Biol, College Pk, MD 20742 USA
Titolo Testata:
PLANT JOURNAL
fascicolo: 2, volume: 28, anno: 2001,
pagine: 237 - 243
SICI:
0960-7412(200110)28:2<237:IVTPAO>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTATHIONE-S-TRANSFERASE; TRANSCRIPTION ACTIVATOR; HYDROGEN-PEROXIDE; SALICYLIC-ACID; IN-VIVO; TOBACCO; GENE; AUXIN; EXPRESSION; ENZYMES;
Keywords:
TGA1a; as-1; chromatin immunoprecipitation; glutathione S-transferase; xenobiotic stress;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Arias, J Univ Maryland, Inst Biotechnol, Agr Biotechnol Ctr, 5115 Plant Sci Bldg, College Pk, MD 20742 USA Univ Maryland 5115 Plant Sci Bldg College Pk MD USA 20742 742 USA
Citazione:
C. Johnson et al., "In vivo target promoter-binding activities of a xenobiotic stress-activated TGA factor", PLANT J, 28(2), 2001, pp. 237-243

Abstract

Xenobiotic chemicals induce the expression of nuclear detoxification genes. A full understanding of this protective response will require characterization of its transcriptional regulatory machinery. We describe here the useof a recently developed plant chromatin immunoprecipitation (ChIP) assay to define nuclear promoter targets of TGA1a, a tobacco basic/leucine zipper transcription factor whose activity is potentiated by herbicide-induced xenobiotic stress. TGA1a selectively binds as-1-type cis-elements, which regulate transcription of putative detoxification and defense genes. With ChIP, we show that endogenous TGA1a binds as-1-containing promoter sequences of two tobacco glutathione S-transferase genes, GNT1 and GNT35. This binding activity is strongly enhanced by xenobiotic stress, as is expression of thesegenes. In contrast, TGA1a apparently does not bind in vivo to functional as-1 elements in promoters of PR-1a and PG13, genes whose expression is insensitive to this stimulus. The findings here thus discriminate between a number of possible functional promoter binding sites for a trans-regulatory factor, within the context of a signal response pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:43:53