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Titolo:
Altered gene expression profile in chemically induced rat mammary adenocarcinomas and its modulation by an aromatase inhibitor
Autore:
Wang, Y; Hu, L; Yao, RS; Wang, M; Crist, KA; Grubbs, CJ; Johanning, GL; Lubet, RA; You, M;
Indirizzi:
Ohio State Univ, Ctr Comprehens Canc, Div Human Canc Genet, Sch Publ Hlth,Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 ch Publ Hlth,Columbus, OH 43210 USA Med Coll Ohio, Toledo, OH 43614 USA Med Coll Ohio Toledo OH USA 43614Med Coll Ohio, Toledo, OH 43614 USA Ohio State Univ, Div Human Canc Genet, Ctr Comprehens Canc, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 rehens Canc, Columbus, OH 43210 USA Univ Alabama, Chemoprevent Ctr, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 revent Ctr, Birmingham, AL 35294 USA Univ Alabama, Dept Nutr Sci, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 t Nutr Sci, Birmingham, AL 35294 USA NCI, Chemoprevent Branch, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892 CI, Chemoprevent Branch, Bethesda, MD 20892 USA
Titolo Testata:
ONCOGENE
fascicolo: 53, volume: 20, anno: 2001,
pagine: 7710 - 7721
SICI:
0950-9232(20011122)20:53<7710:AGEPIC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING PROTEIN-1 CONCENTRATION; BREAST-CANCER; CELL-CYCLE; VOROZOLE R-83842; OVARIAN-CANCER; DNA MICROARRAY; CARCINOMA; RNA; D1; TUMORS;
Keywords:
rat; mammary; adenocarcinoma; gene expression; competitive cDNA library screening; vorozole; chemoprevention;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: You, M Ohio State Univ, Ctr Comprehens Canc, Div Human Canc Genet, Sch Publ Hlth,Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 Hlth,Columbus, OH 43210 USA
Citazione:
Y. Wang et al., "Altered gene expression profile in chemically induced rat mammary adenocarcinomas and its modulation by an aromatase inhibitor", ONCOGENE, 20(53), 2001, pp. 7710-7721

Abstract

In the present study, competitive cDNA library screening (CCLS) and cDNA microarray analyses were employed to identify differentially expressed genesin methylnitrosourea-induced rat mammary adenocarcinomas. The preliminary screening of 100 000 plaques by CCLS identified 1217 clones with differential expression. Dot-blot analysis of the isolated clones verified differential expression in 471 distinct genes. Confirmation of these 471 genes was conducted by performing reverse transcription-polymerase chain reactions, anda total of 160 genes were confirmed after comparing six rat mammary adenocarcinomas and three normal rat mammary glands. Fifty-nine of these showed lower expression in the adenocarcinomas while the remaining 101 were overexpressed in the tumors. Employing a cDNA microarray containing 588 known genes revealed an additional 33 differentially expressed genes in these tumors. Importantly, most of the identified genes demonstrated relatively reproducible overexpression or underexpression in individual tumors. Many of the altered genes determined by cDNA microarray analysis were oncogenes, tumor suppressor genes, or genes involved in cell cycle control and apoptosis. CCLSidentified many others not previously associated with mammary carcinogenesis, including a novel gene named RMT-7. Preliminary studies to determine the applicability of this gene expression approach for detecting potential biomarkers for cancer chemoprevention was evaluated in rat mammary tumors obtained from animals treated with vorozole, a potent aromatase inhibitor. When genes exhibiting differential expression as determined by CCLS or cDNA microarray analysis were examined in control and vorozole-treated tumors, expression of 19 genes was found to be modulated significantly in tumors treated with vorozole. Further investigations into these identified genes shouldcontribute significantly to our understanding of the molecular mechanisms of rat mammary tumorigenesis. In addition, the identified genes may become useful targets for drug development and potential biomarkers for monitoringtreatment and prevention of breast cancer in humans.

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Documento generato il 10/07/20 alle ore 09:38:32