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Titolo:
Kinesin-dependent movement on microtubules precedes actin-based motility of vaccinia virus
Autore:
Rietdorf, J; Ploubidou, A; Reckmann, I; Holmstrom, A; Frischknecht, F; Zettl, M; Zimmermann, T; Way, M;
Indirizzi:
European Mol Biol Lab, D-69117 Heidelberg, Germany European Mol Biol Lab Heidelberg Germany D-69117 117 Heidelberg, Germany
Titolo Testata:
NATURE CELL BIOLOGY
fascicolo: 11, volume: 3, anno: 2001,
pagine: 992 - 1000
SICI:
1465-7392(200111)3:11<992:KMOMPA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENTEROPATHOGENIC ESCHERICHIA-COLI; EXTRACELLULAR ENVELOPED VIRUS; ALDRICH-SYNDROME PROTEIN; TRANS-GOLGI NETWORK; AXONAL-TRANSPORT; ANTEROGRADE TRANSPORT; TAIL FORMATION; MEMBRANE; PHOSPHORYLATION; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Way, M Imperial Canc Res Fund, 44 Lincolns Inn Fields, London WC2A 3PX, England Imperial Canc Res Fund 44 Lincolns Inn Fields London England WC2A 3PX
Citazione:
J. Rietdorf et al., "Kinesin-dependent movement on microtubules precedes actin-based motility of vaccinia virus", NAT CELL BI, 3(11), 2001, pp. 992-1000

Abstract

Vaccinia virus, a close relative of the causative agent of smallpox, exploits actin polymerization to enhance its cell-to-cell spread. We show that actin-based motility of vaccinia is initiated only at the plasma membrane and remains associated with it. There must therefore be another form of cytoplasmic viral transport, from the cell centre, where the virus replicates, to the periphery. Video analysis reveals that GFP-labelled intracellular enveloped virus particles (IEVs) move from their perinuclear site of assembly to the plasma membrane on microtubules. We show that the viral membrane protein A36R, which is essential for actin-based motility of vaccinia, is alsoinvolved in microtubule-mediated movement of IEVs. We further show that conventional kinesin is recruited to IEVs via the light chain TPR repeats andis required for microtubule-based motility of the virus. Vaccinia thus sequentially exploits the microtubule and actin cytoskeletons to enhance its cell-to-cell spread.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 15:41:36