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Titolo:
Delivery of herpes simplex virus vectors through liposome formulation
Autore:
Fu, XP; Zhang, XL;
Indirizzi:
Baylor Coll Med, Ctr Cell & Gene Therapy, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 Gene Therapy, Houston, TX 77030 USA Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 , Dept Pediat, Houston, TX 77030 USA Baylor Coll Med, Dept Mol Virol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 ept Mol Virol, Houston, TX 77030 USA Baylor Coll Med, Dept Microbiol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 ept Microbiol, Houston, TX 77030 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 5, volume: 4, anno: 2001,
pagine: 447 - 453
SICI:
1525-0016(200111)4:5<447:DOHSVV>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMORAL IMMUNE-RESPONSES; GENE-TRANSFER; ADENOVIRAL VECTORS; RIBONUCLEOTIDE REDUCTASE; PREEXISTING IMMUNITY; MALIGNANT GLIOMAS; TUMOR-THERAPY; VIRAL THERAPY; BRAIN-TUMORS; IN-VITRO;
Keywords:
HSV; vectors; liposome; BAC; systemic delivery; immune response; capsids; oncolytic;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Zhang, XL Baylor Coll Med, Ctr Cell & Gene Therapy, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 apy, Houston, TX 77030 USA
Citazione:
X.P. Fu e X.L. Zhang, "Delivery of herpes simplex virus vectors through liposome formulation", MOL THER, 4(5), 2001, pp. 447-453

Abstract

Viral vectors have been widely used as gene delivery vehicles for both experimental and clinical investigations. Although these vectors are capable of achieving high gene transduction efficiency in vitro, one of the major limitations facing the therapeutic viral vectors is that the preexisting hostanti-vector immunity can substantially reduce their transduction efficiency in vivo. This is especially of concern when the therapeutic remedy requires repeated systemic administration. Here we report the delivery of herpes simplex virus (HSV) derived vectors through liposome formulation. In these studies, we have prepared HSV vectors in three different forms for liposomeformulation: purified viral DNA (obtained from a bacterial artificial chromosome containing an infectious HSV genome), HSV capsids, and intact viral particles. All three forms of HSV were readily transfected into cultured cells and infectious virus was efficiently generated. Furthermore, introduction of HSV vectors as DNA/liposome complexes improved in vivo transduction efficiency, by effectively evading the host anti-HSV immunity during systemic administration. We conclude that viral vectors such as HSV can be systemically delivered through liposome formulation for safe and repeated administration for gene transduction or oncolytic purposes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/02/20 alle ore 21:56:23