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Titolo:
Electroporation-facilitated delivery of plasmid DNA in skeletal muscle: Plasmid dependence of muscle damage and effect of poloxamer 188
Autore:
Hartikka, J; Sukhu, I; Buchner, C; Hazard, D; Bozoukova, V; Margalith, M; Nishioka, WK; Wheeler, CJ; Manthorpe, M; Sawdey, M;
Indirizzi:
Vical Inc, San Diego, CA 92121 USA Vical Inc San Diego CA USA 92121Vical Inc, San Diego, CA 92121 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 5, volume: 4, anno: 2001,
pagine: 407 - 415
SICI:
1525-0016(200111)4:5<407:EDOPDI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VIVO ELECTROPORATION; DIRECT GENE-TRANSFER; INTRAMUSCULAR INJECTION; HIGH-LEVEL; EXPRESSION; THERAPY; MOUSE; ELECTROCHEMOTHERAPY; ELECTROTRANSFER; EFFICACY;
Keywords:
electroporation; naked DNA; skeletal muscle; poloxamer 188; pluronic F68;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Sawdey, M Vical Inc, 9373 Towne Ctr Dr,Suite 100, San Diego, CA 92121 USA Vical Inc 9373 Towne Ctr Dr,Suite 100 San Diego CA USA 92121 USA
Citazione:
J. Hartikka et al., "Electroporation-facilitated delivery of plasmid DNA in skeletal muscle: Plasmid dependence of muscle damage and effect of poloxamer 188", MOL THER, 4(5), 2001, pp. 407-415

Abstract

Electroporation has been reported to facilitate naked DNA gene transfer inskeletal muscle, but has also been implicated in the pathogenesis of electrical injuries. To assess the effects of electroporation on gene transfer, mouse quadriceps muscles were injected with the luciferase reporter plasmidVR1255 and electroporated with caliper electrodes. Intramuscular luciferase expression was increased 10- to 70-fold by electroporation, depending on the DNA dose and injection volume used. In the absence of plasmid DNA injection, electroporation of quadriceps muscles resulted in rapid elevations inserum creatine phosphokinase activity, but did not elicit visible muscle damage. However, in muscles injected with plasmid DNA and electroporated, visible lesions consistently developed in the areas proximal to electrode placement when field strengths optimal for gene transfer (300 volts/cm) were applied. The development of muscle lesions was independent of plasmid transgene expression and required the presence of plasmid in the muscle during electroporation. Co-injection of poloxamer 188 (pluronic F68) with VR1255 substantially reduced elevations in serum creatine phosphokinase activity following electroporation, but did not inhibit the development of muscle lesions. In non-electroporated muscles, co-injection of poloxamer 188 increased luciferase expression threefold. Poloxamer 188 may thus constitute a useful excipient for intramuscular delivery of naked DNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 06:28:33