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Titolo:
Cloning and characterization of the metacyclogenin gene, which is specifically expressed during Trypanosoma cruzi metacyclogenesis
Autore:
Avila, AR; Yamada-Ogatta, SF; Monteiro, VDS; Krieger, MA; Nakamura, CV; de Souza, W; Goldenberg, S;
Indirizzi:
Fiocruz MS, Inst Oswaldo Cruz, Dept Bioquim & Biol Mol, BR-21045900 Rio DeJaneiro, Brazil Fiocruz MS Rio De Janeiro Brazil BR-21045900 BC900 Rio DeJaneiro, Brazil Inst Biol Mol Parana, IBMP, TECPAR, BR-81350010 Curitiba, Parana, Brazil Inst Biol Mol Parana Curitiba Parana Brazil BR-81350010 BC Parana, Brazil Univ Estadual Londrina, Londrina, PR, Brazil Univ Estadual Londrina Londrina PR Brazil Londrina, Londrina, PR, Brazil Univ Estadual Maringa, Maringa, Parana, Brazil Univ Estadual Maringa Maringa Parana Brazil nga, Maringa, Parana, Brazil UFRJ, Inst Biofis Carlos Chagas Filho, Rio De Janeiro, Brazil UFRJ Rio DeJaneiro Brazil Carlos Chagas Filho, Rio De Janeiro, Brazil
Titolo Testata:
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
fascicolo: 2, volume: 117, anno: 2001,
pagine: 169 - 177
SICI:
0166-6851(200110)117:2<169:CACOTM>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNAS; DEVELOPMENTAL REGULATION; LEISHMANIA-MAJOR; CYCLIC-AMP; BRUCEI; DIFFERENTIATION; STAGE; PROTEINS; REGION; DNA;
Keywords:
Trypanosoma cruzi; metacyclogenesis; stage-specific gene; differentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Goldenberg, S Fiocruz MS, Inst Oswaldo Cruz, Dept Bioquim & Biol Mol, Avenida Brasil 4365, BR-21045900 Rio De Janeiro, Brazil Fiocruz MS Avenida Brasil 4365 Rio De Janeiro Brazil BR-21045900 BC
Citazione:
A.R. Avila et al., "Cloning and characterization of the metacyclogenin gene, which is specifically expressed during Trypanosoma cruzi metacyclogenesis", MOL BIOCH P, 117(2), 2001, pp. 169-177

Abstract

We isolated a gene that is differentially expressed during Trypanosoma cruzi metacyclogenesis by the representation of differential expression (RDE) method. using differentiating epimastigotes cultured in chemically defined medium. This gene, the metacyclogenin gene, encodes a 630-nucleotide mRNA that is specifically associated with the polysomes of epimastigotes allowed to differentiate for 24 h. We sequenced and characterized the metacyclogenin gene and found that there were at least three copies of the gene organized into tandem 2.8 kb repeats in the genome of T. cruzi Dm28c. We analyzed the repeats and found that they contained two other genes, one encoding tryparedoxin peroxidase and the other encoding a 0.6 kb mRNA (named associated gene or AG) with sequences showing no significant similarity to those in the GenBank database. Northern blot analysis of polysomal RNA extracted from replicating and differentiating epimastigotes showed that metacyclogenin and AG genes displayed similar patterns of expression. Their products were detected only in differentiating epimastigotes, whereas tryparedoxin peroxidase was detected only in the polysomal RNA fraction of replicating and differentiating epimastigotes. In Northern blots of total RNA from differentiating and replicating epimastigotes. the genes studied were detected in both cell populations. The differential expression of the metacyclogenin gene wasconfirmed by immunocytochemistry studies showing that the protein is detected only in differentiating (adhered) epimastigote. The results suggest that mRNA mobilization to polysomes is an important mechanism in the regulation of gene expression in T. cruzi. (C) 2001 Elsevier Science B.V. All rightsreserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 17:23:06