Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A polyketide biosynthetic gene cluster from Streptomyces antibioticus includes a LysR-type transcriptional regulator
Autore:
Colombo, V; Fernandez-de-Heredia, M; Malpartida, F;
Indirizzi:
Univ Autonoma Madrid, Ctr Nacl Biotecnol, E-28049 Madrid, Spain Univ Autonoma Madrid Madrid Spain E-28049 otecnol, E-28049 Madrid, Spain
Titolo Testata:
MICROBIOLOGY-SGM
, volume: 147, anno: 2001,
parte:, 11
pagine: 3083 - 3092
SICI:
1350-0872(200111)147:<3083:APBGCF>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
COELICOLOR A3(2); NUCLEOTIDE-SEQUENCE; SECONDARY METABOLISM; ACTINORHODIN BIOSYNTHESIS; O-METHYLTRANSFERASE; VIOLACEORUBER TU22; HETEROLOGOUS HOST; ESCHERICHIA-COLI; TETRACENOMYCIN-C; DEDUCED FUNCTION;
Keywords:
actll-ORF4; actinorhodin; antibiotic; heterologous complementation; regulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Malpartida, F Univ Autonoma Madrid, Ctr Nacl Biotecnol, E-28049 Madrid, Spain Univ Autonoma Madrid Madrid Spain E-28049 49 Madrid, Spain
Citazione:
V. Colombo et al., "A polyketide biosynthetic gene cluster from Streptomyces antibioticus includes a LysR-type transcriptional regulator", MICROBI-SGM, 147, 2001, pp. 3083-3092

Abstract

In the search for Type II polyketide synthases (PKSs) a DNA fragment was isolated from Streptomyces antibioticus ATCC 11891 (a producer of oleandomycin). DNA sequencing of the cloned fragment revealed six complete Offs whosededuced products showed similarities to those of other genes known to be involved in polyketide biosynthesis. Several S. coelicolor strains mutated in different steps of actinorhodin biosynthesis (actI, actiII, actV(A) and actVII) were complemented by the cloned genes, suggesting that the isolated genes encode an aromatic polyketide of unknown structure and function. The cluster also contains a putative LysR-type transcriptional regulator (ORF0), which controls PKS gene expression in a heterologous host. DNA binding assays and transcriptional analysis suggest that the pathway-specific regulator for actinorhodin biosynthesis (actII-ORF4) is also involved in the expression of the cloned PKS in the host strain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 00:57:25