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Titolo:
Monomer-dimer control of the ColE1 P-cer promoter
Autore:
Chatwin, HM; Summers, DK;
Indirizzi:
Univ Cambridge, Dept Genet, Cambridge CB2 3EH, England Univ Cambridge Cambridge England CB2 3EH net, Cambridge CB2 3EH, England
Titolo Testata:
MICROBIOLOGY-SGM
, volume: 147, anno: 2001,
parte:, 11
pagine: 3071 - 3081
SICI:
1350-0872(200111)147:<3071:MCOTCP>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
SITE-SPECIFIC RECOMBINATION; MULTICOPY PLASMID STABILITY; ESCHERICHIA-COLI; PROTEIN-SYNTHESIS; AMINOPEPTIDASE-A; DNA; TRANSCRIPTION; RESOLUTION; SEQUENCES; REQUIRES;
Keywords:
dimer resolution; cer-Xer recombination system; cell-cycle checkpoint; Red; plasmid stability;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Summers, DK Univ Cambridge, Dept Genet, Downing Site, Cambridge CB2 3EH, England Univ Cambridge Downing Site Cambridge England CB2 3EH England
Citazione:
H.M. Chatwin e D.K. Summers, "Monomer-dimer control of the ColE1 P-cer promoter", MICROBI-SGM, 147, 2001, pp. 3071-3081

Abstract

XerCD-mediated recombination at cer converts multimers of plasmid ColE1 tomonomers, maximizing the number of independently segregating molecules andminimizing the frequency of plasmid loss. In addition to XerCD, recombination requires the accessory factors ArgR and PepA. The promoter P-cer, located centrally within cer, is also required for stable plasmid maintenance. P-cer is active in plasmid multimers and directs transcription of a short RNA, Rcd, which appears to inhibit cell division. It has been proposed that Rcd is part of a checkpoint which ensures that multimer resolution is complete before the cell divides. This study has shown that ArgR does not act as a transcriptional repressor of P-cer in plasmid monomers. P-cer is unusual in that the -35 and -10 hexamers are separated by only 15 bp and this studyhas demonstrated that increasing this to a more conventional spacing results in elevated activity. An increase to 17 bp resulted in a 10- to 20-fold increase in activity, while smaller effects were seen when the spacer was increased to 16 bp or 18 bp. These observations are consistent with the hypothesis that P-cer activation involves realignment of the -35 and -10 sequences within a recombinational synaptic complex. This predicts that a 17 bp spacer promoter derivative should be down-regulated by plasmid multimerization, and this is confirmed experimentally.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 20:13:00