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Titolo:
Repression of XMyoD expression and myogenesis by Xhairy-1 in Xenopus earlyembryo
Autore:
Umbhauer, M; Boucaut, JC; Shi, DL;
Indirizzi:
Univ Paris 06, CNRS, UMR 7622, Lab Biol Dev,Grp Biol Expt, F-75005 Paris, France Univ Paris 06 Paris France F-75005 ,Grp Biol Expt, F-75005 Paris, France
Titolo Testata:
MECHANISMS OF DEVELOPMENT
fascicolo: 1, volume: 109, anno: 2001,
pagine: 61 - 68
SICI:
0925-4773(200111)109:1<61:ROXEAM>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYOD EXPRESSION; MESODERM INDUCTION; EARLY RESPONSE; NOTCH; SEGMENTATION; GENE; HAIRY; CELLS; DOMAIN; SPLIT;
Keywords:
MyoD; Myf5; myogenic factor; myogenesis; mesoderm; notch signalling; hairy; repressor; Xenopus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Shi, DL Univ Paris 06, CNRS, UMR 7622, Lab Biol Dev,Grp Biol Expt, 9 Quai St Bernard, F-75005 Paris, France Univ Paris 06 9 Quai St Bernard Paris France F-75005 aris, France
Citazione:
M. Umbhauer et al., "Repression of XMyoD expression and myogenesis by Xhairy-1 in Xenopus earlyembryo", MECH DEVEL, 109(1), 2001, pp. 61-68

Abstract

Activated Notch-Delta signalling was shown to inhibit myogenesis, but whether and how it regulates myogenic gene expression is not clear. We analyzedthe implication of Xenopus hairy-1 (Xhairy-1), a member of the hairy and enhancer-of-split (E(spl)) family that may function as nuclear effector of Notch signalling pathway, in regulating XMyoD gene expression at the initialstep of myogenesis. Xhairy-1 transcripts are expressed soon after mid-blastula transition and exhibits overlapping expression with Notch pathway genes such as Delta-1 in the posterior somitic mesoderm. We show that overexpression of Xhairy-1 blocks the expression of XMyoD in early gastrula ectodermal cells treated with the mesoderm-inducing factor activin, and in the mesoderm tissues of early embryos. It inhibits myogenesis and produces trunk defects at later stages. Xhairy-1 also inhibits the expression of the pan-mesodermal marker Xbra, but expression of other early mesoderm markers such asgoosecoid and chordin is not affected. These effects require the basic helix-loop-helix (bHLH) domain, as well as a synergy between the central Orange domain and the C-terminus WRPW-Groucho-interacting domain. Furthermore, overexpression in ectodermal cells of Xhairy-1/VP16, in which Xhairy-1 repressor domain is replaced by the activator domain of the viral protein VP16, induces the expression of XMyoD in the absence of protein synthesis. Interestingly, Xhairy-1/VP16 does not induce the expression of Xbra and XMyf5 in the same condition. During neurulation, the expression of XMyoD induced by Xhairy-1/VP16 declines and the expression of muscle actin gene was never detected. These results suggest that Notch signalling through hairy-related genes may specifically regulate XMyoD expression at the initial step of myogenesis in vertebrates. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 00:45:07