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Titolo:
Vascular endothelial growth factor production by isolated rat hepatocytes after cold ischemia-warm reoxygenation
Autore:
Archambault, AJ; Sirois, MG; Bernatchez, PN; Fiset, C; Haddad, PS;
Indirizzi:
Univ Montreal, Dept Pharmacol, Montreal, PQ H3C 3J7, Canada Univ MontrealMontreal PQ Canada H3C 3J7 ol, Montreal, PQ H3C 3J7, Canada Univ Montreal, Membrane Transport Res Grp, Montreal, PQ H3C 3J7, Canada Univ Montreal Montreal PQ Canada H3C 3J7 rp, Montreal, PQ H3C 3J7, Canada Montreal Heart Inst, Montreal, PQ H1T 1C8, Canada Montreal Heart Inst Montreal PQ Canada H1T 1C8 ntreal, PQ H1T 1C8, Canada
Titolo Testata:
LIVER TRANSPLANTATION
fascicolo: 11, volume: 7, anno: 2001,
pagine: 988 - 997
SICI:
1527-6465(200111)7:11<988:VEGFPB>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYPOTHERMIC PRESERVATION; LIVER-TRANSPLANTATION; REPERFUSION INJURY; KINASE ACTIVATION; PROTEIN-SYNTHESIS; RISK-FACTORS; CELLS; MECHANISMS; MODULATION; VIABILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Haddad, PS Univ Montreal, Dept Pharmacol, POB 6128,Downtown Stn, Montreal,PQ H3C 3J7, Canada Univ Montreal POB 6128,Downtown Stn Montreal PQ Canada H3C 3J7
Citazione:
A.J. Archambault et al., "Vascular endothelial growth factor production by isolated rat hepatocytes after cold ischemia-warm reoxygenation", LIVER TRANS, 7(11), 2001, pp. 988-997

Abstract

Inflammatory disturbances in the liver microcirculation have been associated with preservation injury of hepatic grafts. Vascular endothelial growth factor (VEGF), a proinflammatory growth factor released by hepatocytes, acts on sinusoidal endothelial cells, but its implication in graft injury is stiff unclear. We studied VEGF production by rat hepatocytes after cold ischemia and warm reoxygenation and compared the capacity of University of Wisconsin (UW) and sodium-lactobionate-sucrose (SLS) preservation solutions to maintain this hepatocellular function. Isolated hepatocytes were kept for 0, 24, and 48 hours at 4 degreesC in either solution (cold ischemia), then incubated for 1 to 24 hours at 37 degreesC (warm reoxygenation). We assessedcell viability and production of VEGF messenger RNA (mRNA) and protein. Cell viability decreased in a linear time-dependent fashion by 10% after 48 hours of cold preservation and by an additional 40% after 24 hours of warm culture. Very little VEGF mRNA could be detected after up to 48 hours of simple cold preservation in either solution. However, subsequent warm culture led to a robust and rapid increase in VEGF mRNA expression within the firsthour, which declined to close to background levels within 8 to 12 hours inculture. This effect was more important in cells preserved in SLS than UW solution. Similarly, cold preservation alone did not trigger VEGF secretion. VEGF secretion was detected after culturing hepatocytes at 37 degreesC and reached a maximal secretion rate within 12 to 15 hours. However, VEGF production by preserved cells was reduced compared with unstored cells. In conclusion, cold ischemia and warm reoxygenation triggers VEGF mRNA expressionby hepatocytes, but subsequent VEGF secretion is partially impaired, suggesting posttranslational defects.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 12:21:45