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Titolo:
cDNA cloning of feline leptin and its mRNA expression in adipose tissue
Autore:
Sasaki, N; Shibata, H; Honjoh, T; Kimura, K; Saito, M; Ohishi, I;
Indirizzi:
Nippon Vet & Anim Sci Univ, Dept Vet Sci, Div Vet Biochem, Musashino, Tokyo 1808602, Japan Nippon Vet & Anim Sci Univ Musashino Tokyo Japan 1808602 o 1808602, Japan Morinaga & Co Ltd, Res Inst, Tsurumi Ku, Yokohama, Kanagawa 2308504, JapanMorinaga & Co Ltd Yokohama Kanagawa Japan 2308504 Kanagawa 2308504, Japan Hokkaido Univ, Grad Sch Vet Med, Dept Biomed Sci, Sapporo, Hokkaido 0600818, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600818 oro, Hokkaido 0600818, Japan
Titolo Testata:
JOURNAL OF VETERINARY MEDICAL SCIENCE
fascicolo: 10, volume: 63, anno: 2001,
pagine: 1115 - 1120
SICI:
0916-7250(200110)63:10<1115:CCOFLA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
OBESE GENE-PRODUCT; BODY-WEIGHT; OB PROTEIN; HUMANS; PLASMA; MOUSE; MICE; FAT; RNA; HYPOTHALAMUS;
Keywords:
adipose tissue; cDNA cloning; feline; leptin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Sasaki, N Nippon Vet & Anim Sci Univ, Dept Vet Sci, Div Vet Biochem, Musashino, Tokyo 1808602, Japan Nippon Vet & Anim Sci Univ Musashino Tokyo Japan 1808602 Japan
Citazione:
N. Sasaki et al., "cDNA cloning of feline leptin and its mRNA expression in adipose tissue", J VET MED S, 63(10), 2001, pp. 1115-1120

Abstract

Leptin, the product of the obese (ob) gene. is an adipocyte-derived hormone involved in regulating food intake and energy expenditure in humans and rodents. To determine the primary structure of feline leptin, we cloned the feline leptin cDNA using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of complementary DNA (cDNA) ends (RACE) methods. The full-length feline leptin cDNA was 2935 bp with a 501 bp open reading frame encoding the precursor peptide of 167 amino acids including 21 residues of signal peptide. The sequence of a 146-amino acid mature leptin was 81.5-91.8% homologous to those of other species. RT-PCR analysis revealedthat the leptin mRNA was expressed in adipose tissues and not detected in liver, hearth kidney, lung, pancreas, brain and skeletal muscle. These datashow that feline leptin is highly homologous to leptins of other species. and expressed in adipose tissues in cats.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 16:20:23