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Titolo:
Alterations in gap junction protein expression in human benign prostatic hyperplasia and prostate cancer
Autore:
Habermann, H; Ray, V; Habermann, W; Prins, GS;
Indirizzi:
Univ Illinois, Dept Urol, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 nois, Dept Urol, Chicago, IL 60612 USA Provident Hosp, Dept Pathol, Chicago, IL USA Provident Hosp Chicago IL USA ovident Hosp, Dept Pathol, Chicago, IL USA Graz Univ, Dept Urol, Graz, Austria Graz Univ Graz AustriaGraz Univ, Dept Urol, Graz, Austria Graz Univ, Dept Otorhinolaryngol Head & Neck Surg, Graz, Austria Graz Univ Graz Austria Otorhinolaryngol Head & Neck Surg, Graz, Austria
Titolo Testata:
JOURNAL OF UROLOGY
fascicolo: 6, volume: 166, anno: 2001,
pagine: 2267 - 2272
SICI:
0022-5347(200112)166:6<2267:AIGJPE>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-CELL COMMUNICATION; INTERCELLULAR COMMUNICATION; CONNEXIN-43 EXPRESSION; TUMOR-CELLS; GENE; LOCALIZATION; GROWTH; PHOSPHORYLATION; ESTROGEN;
Keywords:
prostate; prostatic neoplasms; prostatic hyperplasia; GTPase-activating proteins; connexins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Prins, GS Univ Illinois, Dept Urol, M-C 955, Chicago, IL 60612 USA Univ Illinois M-C 955 Chicago IL USA 60612 Chicago, IL 60612 USA
Citazione:
H. Habermann et al., "Alterations in gap junction protein expression in human benign prostatic hyperplasia and prostate cancer", J UROL, 166(6), 2001, pp. 2267-2272

Abstract

Purpose: Gap junctions composed of connexin proteins have an essential role in intercellular communication and differentiation. Dysregulation of connexin expression is believed to have a role in carcinogenesis. The human prostate has been reported to express connexin 32 and 43. However, the expression pattern in prostate cancer is controversial, while to our knowledge connexin expression has not been reported in benign prostatic hyperplasia (BPH). To understand the potential involvement in prostate disease connexin 32 and 43 expression was evaluated in a series of normal prostate, BPH and prostate cancer specimens that were surgically removed due to bladder outlet obstruction. Materials and Methods: Frozen sections of 23 normal, 43 BPH and 40 cancer involved prostates were evaluated for the presence, staining intensity and pattern of connexin 32 and 43 by immunocytochemical testing. Results: In all specimens examined connexin 43 stain was punctate along the borders of the basal epithelial cells, whereas connexin 32 immunolocalized to luminal epithelial cells: In normal prostate connexin 43 and 32 were present in 87% and 65% of specimens, respectively, at low to moderate stain intensity. Importantly none of the normal samples were negative for each connexin. In BPH specimens there was a marked increase in the incidence and intensity of connexin 43 and 32 immunostaining within epithelial cells. In addition, 23% of BPH samples showed strong connexin 43 expression in stromalcells. In contrast, connexin was decreased in prostate cancer specimens, of which 65% and 38% were negative for connexin 43 and 32, respectively, and28% were negative for each type. In poorly differentiated tumors connexin 43 and 32 were present in only 10% and 40% of tumors, respectively, at low immunostaining intensity. Conclusions: In normal human prostate basal cells communicate via connexin43 gap junctions, whereas luminal cells communicate via connexin 32 gap junctions. In BPH gap junctional intercellular communication is increased in epithelial and stromal cells, which may have a role in BPH pathogenesis. Inprostate cancer gap junctional intercellular communication is decreased, is as indicated by decreased expression of connexin 43 and 32 with severe loss in poorly differentiated prostate cancer. These alterations in connexin expression may have a role in dedifferentiation and tumor progression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:46:54