Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Pre-treatment of Caco-2 cells with zinc during the differentiation phase alters the kinetics of zinc uptake and transport
Autore:
Reeves, PG; Briske-Anderson, M; Johnson, LA;
Indirizzi:
USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA USDA ARS Grand Forks ND USA 58202 Nutr Res Ctr, Grand Forks, ND 58202 USA
Titolo Testata:
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
fascicolo: 12, volume: 12, anno: 2001,
pagine: 674 - 684
SICI:
0955-2863(200112)12:12<674:POCCWZ>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
BORDER MEMBRANE-VESICLES; RICH INTESTINAL PROTEIN; RAT INTESTINE; DIETARY ZINC; METALLOTHIONEIN; ABSORPTION; MODEL; MECHANISMS;
Keywords:
Caco-2 cells; zinc transport; transport kinetics; zinc uptake; absorption; trace elements;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Reeves, PG USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202USA USDA ARS Grand Forks ND USA 58202 r, Grand Forks, ND 58202 USA
Citazione:
P.G. Reeves et al., "Pre-treatment of Caco-2 cells with zinc during the differentiation phase alters the kinetics of zinc uptake and transport", J NUTR BIOC, 12(12), 2001, pp. 674-684

Abstract

The Caco-2 cell model was used to study the efficiency of absorption and endogenous excretion of zinc (Zn) regulated by dietary Zn concentration. Cells were seeded onto high pore-density membranes and maintained in medium supplemented with 10% FBS. After confluence. cells were treated with 5 or 25 mu mol Zn/L for 7 d, and Zn uptake and transport were measured in both apical (AP) and basolateral (BL) directions by using Zn-65. Similar cells were labeled with Zn-65 and the release of Zn to the AP and BL sides was measured. The AP uptake of Zn in cells exposed to 25 mu mol Zn/L was slower (p < 0.05) than that in cells exposed to 5 mu mol Zn/L. The AP to BL transport rate in the 25 mu mol Zn/L group was only 40% (p < 0.05) of that in the 5 muMgroup. In contrast, the rate of BL Zn uptake was 4-fold higher in cells treated with 25 mu mol Zn/L than in those treated with 5 mu mol Zn/L (p < 0.05). The BL to AP transport rate was 2-fold higher in cells treated with 25 mu mol Zn/L than in those treated with 5 mu mol Zn/L (p < 0.05). Basolateral uptake was 6 to 25 times greater (p < 0.05) than AP uptake for cells treated with 5 and 25 mu mol Zn/L, respectively. The rate of Zn release was enhanced about 4-fold (p < 0.05) by 25 mu mol Zn/L treatment. Release to the BL side was 10 times greater than to the AP side. Zn-induced metallothionein(MT), thought to down-regulate AP to BL Zn transport, was 4-fold higher (p< 0.001) in the 25 mu mol Zn/L group than in the 5 muM group, but the rateof BL Zn release was higher in cells treated with 25 mu mol Zn/L than in those treated with 5 mu mol Zn/L (p < 0.05). Induced changes in transport rates by media Zn concentrations could involve the up- and/or down-regulationof Zn influx and efflux proteins such as the ZIP and ZnT families of Zn transporters. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 17:03:01