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Titolo:
Monitoring of reactive oxygen species production after traumatic brain injury in rats with microdialysis and the 4-hydroxybenzoic acid trapping method
Autore:
Marklund, N; Clausen, F; Lewander, T; Hillered, L;
Indirizzi:
Univ Uppsala Hosp, Dept Neurosci & Neurosurg, SE-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden SE-75185 surg, SE-75185 Uppsala, Sweden Univ Uppsala Hosp, Dept Psychiat, SE-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden SE-75185 hiat, SE-75185 Uppsala, Sweden Univ Uppsala Hosp, Dept Med Sci, SE-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden SE-75185 Sci, SE-75185 Uppsala, Sweden
Titolo Testata:
JOURNAL OF NEUROTRAUMA
fascicolo: 11, volume: 18, anno: 2001,
pagine: 1217 - 1227
SICI:
0897-7151(200111)18:11<1217:MOROSP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPRESSION CONTUSION TRAUMA; HYDROXYL RADICAL GENERATION; EXPERIMENTAL HEAD-INJURY; LIPID-PEROXIDATION; CAUTIONARY NOTE; SODIUM-SALICYLATE; OXIDATIVE STRESS; CELL-DEATH; ISCHEMIA; RELEASE;
Keywords:
free radicals; 2-hydroxybenzoic acid (salicylate : 2-HBA); 4-hydroxybenzoic acid (4-HBA); microdialysis; reactive oxygen species; traumatic brain injury (TBI);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Marklund, N Univ Uppsala Hosp, Dept Neurosci & Neurosurg, SE-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden SE-75185 85 Uppsala, Sweden
Citazione:
N. Marklund et al., "Monitoring of reactive oxygen species production after traumatic brain injury in rats with microdialysis and the 4-hydroxybenzoic acid trapping method", J NEUROTRAU, 18(11), 2001, pp. 1217-1227

Abstract

The detection of reactive oxygen species (ROS) after traumatic brain injury (TBI) is based on indirect methods due to the high reactivity and short half-life of ROS in biological tissue. The commonly used salicylate trappingmethod has several disadvantages making it unsuitable for human use. We have evaluated 4-hydroxybenzoic acid (4-HBA) together with microdialysis (MD)in the rat as an alternative method. 4-HBA forms one stable adduct, 3,4-dihydroxybenzoic acid (3,4-DHBA), when reacting with ROS and has not previously been used together with MD after TBI. Twenty-seven rats were used for the assessment of 3,4-DHBA production as an indicator of ROS formation in a controlled contusion injury model using intracerebral MD with 3 mM 4-HBA in the perfusate. For comparison, salicylate trapping was used in eight rats. TBI caused a 250 % increase of 3,4-DHBA that peaked at 30 min after injury in severely injured rats and remained significantly elevated as compared tobaseline for 90 min after trauma. The mild injury level caused a 100% increase in 3,4-DHBA formation at 30 min after the injury. When the MD probe was placed in the perimeter of the injury site, no significant increase in ROS formation occurred. Salicylate trapping showed a similar increase in adduct formation after severe injury. In addition, high cortical concentrationsof 4-HBA and salicylate were found. It is concluded that microdialysis with 4-HBA as a trapping agent appears to be a useful method for ROS detectionin the rat with a potential clinical utility.

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Documento generato il 03/06/20 alle ore 09:47:14