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Titolo:
The role of CD44 during CD40 ligand-induced dendritic cell clustering and maturation
Autore:
Termeer, C; Johannsen, H; Braun, T; Renkl, A; Ahrens, T; Denfeld, RW; Lappin, MB; Weiss, JM; Simon, JC;
Indirizzi:
Univ Freiburg, Dept Dermatol, D-79104 Freiburg, Germany Univ Freiburg Freiburg Germany D-79104 rmatol, D-79104 Freiburg, Germany Bioctr Basel, Dept Biophys Chem, Basel, Switzerland Bioctr Basel Basel Switzerland l, Dept Biophys Chem, Basel, Switzerland Univ Glasgow, Dept Med, Acad Transfus Med Unit, Glasgow G12 8QQ, Lanark, Scotland Univ Glasgow Glasgow Lanark Scotland G12 8QQ ow G12 8QQ, Lanark, Scotland
Titolo Testata:
JOURNAL OF LEUKOCYTE BIOLOGY
fascicolo: 5, volume: 70, anno: 2001,
pagine: 715 - 722
SICI:
0741-5400(200111)70:5<715:TROCDC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MHC CLASS-II; T-CELLS; IN-VIVO; HYALURONAN-BINDING; CD40-CD40 LIGAND; HUMAN MONOCYTES; CROSS-LINKING; CUTTING EDGE; B-CELLS; ACTIVATION;
Keywords:
hyaluronic acid; MHC; TNF-alpha; IL-1 beta; CD154;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Termeer, C Univ Freiburg, Dept Dermatol, Hauptstr 7, D-79104 Freiburg, Germany Univ Freiburg Hauptstr 7 Freiburg Germany D-79104 urg, Germany
Citazione:
C. Termeer et al., "The role of CD44 during CD40 ligand-induced dendritic cell clustering and maturation", J LEUK BIOL, 70(5), 2001, pp. 715-722

Abstract

The interaction between CD40 on dendritic cells (DC) and its ligand CD154 has been recognized to be an important feature in the maturation of DC. Here, we were interested in the role of CD44 a surface receptor shown to mediate cell-cell adhesion and binding to Hyaluronic acid (HA). Western blot analysis of human DC stimulated for 3-12 h with CD 154 revealed the rapid induction of the 85 kDa standard form of CD44 and an increased HA-binding affinity. Time-lapse video-imaging microscopy of human DC co-cultured on CD154-transfected murine fibroblasts showed that the CD44 up-regulation coincided with the rapid induction of homotypic DC clustering, which did not occur onempty vector-transfected fibroblasts. In this system, addition of anti-CD44s mAbs abrogated DC-cluster formation, thereby inhibiting further maturation, as shown by a reduced TNF-a. production and inhibition of CD 154-induced MHC class II up-regulation. However, co-incubation with HA-degrading enzymes induced no changes in the CD 154-mediated DC clustering and maturation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 14:50:48