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Titolo:
Evidence for sodium-coupled acid-base transport across the basolateral membrane of the reabsorptive duct of the human eccrine sweat gland
Autore:
Beck, JS; Coulson, HF; Dove, N; Kealey, T;
Indirizzi:
Unilever Res Port Sunlight Lab, Wirral L63 3JW, Merseyside, England Unilever Res Port Sunlight Lab Wirral Merseyside England L63 3JW England Univ Cambridge, Dept Clin Biochem, Cambridge, England Univ Cambridge Cambridge England Dept Clin Biochem, Cambridge, England
Titolo Testata:
JOURNAL OF INVESTIGATIVE DERMATOLOGY
fascicolo: 4, volume: 117, anno: 2001,
pagine: 877 - 879
SICI:
0022-202X(200110)117:4<877:EFSATA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-VOLUME; H+; NA+;
Keywords:
Na : H exchange; pH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Beck, JS Unilever Res Port Sunlight Lab, Quarry Rd E, Wirral L63 3JW, Merseyside, England Unilever Res Port Sunlight Lab Quarry Rd E Wirral Merseyside England L63 3JW
Citazione:
J.S. Beck et al., "Evidence for sodium-coupled acid-base transport across the basolateral membrane of the reabsorptive duct of the human eccrine sweat gland", J INVES DER, 117(4), 2001, pp. 877-879

Abstract

Intracellular pH was measured in isolated nonperfused ducts of human eccrine sweat glands in vitro to investigate basolateral acid-base transport mechanisms. Bath sodium removal led to a bicarbonate-independent, 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid insensitive acidification. The recovery of this acidification was ethylisopropyl amiloride sensitive, suggestive of basolateral sodium:hydrogen exchange. Whereas bath chloride removal led to a small acidification this was not 4-acetamido-4-isothiocyanatostilbene-2,2'-disulfonic acid sensitive and its causes remain unclear. Elevation of bath potassium to depolarize the basolateral membrane led to a small alkalinization but this was not mimicked by addition of barium or chloride removal. As chloride removal and barium addition would be expected to cause larger depolarizations than potassium elevation these observations do not support a major role for electrogenic acid-base transport. In conclusion, although this study does not support a major role for electrogenic acid-base transport, it has demonstrated the basolateral presence of sodium-coupled acid-base transport in the reabsorptive duct of the human eccrine sweatgland, which most likely represents a sodium:hydrogen exchanger involved in regulation of intracellular pH.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 15:51:09