Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of intramyocardial injection of phVEGF-A(165) as sole therapy in patients with refractory coronary artery disease - 12-month follow-up: Angiogenic gene therapy
Autore:
Sarkar, N; Ruck, A; Kallner, G; Y-Hassan, S; Blomberg, P; Islam, KB; Van Der Linden, J; Lindblom, D; Nygren, AT; Lind, B; Brodin, LA; Drvota, V; Sylven, C;
Indirizzi:
Huddinge Univ Hosp, Karolinska Inst, Dept Cardiol, S-14186 Huddinge, Sweden Huddinge Univ Hosp Huddinge Sweden S-14186 iol, S-14186 Huddinge, Sweden Huddinge Univ Hosp, Karolinska Inst, Dept Thorac Surg, S-14186 Huddinge, Sweden Huddinge Univ Hosp Huddinge Sweden S-14186 urg, S-14186 Huddinge, Sweden Huddinge Univ Hosp, Karolinska Inst, Dept Clin Physiol, S-14186 Huddinge, Sweden Huddinge Univ Hosp Huddinge Sweden S-14186 iol, S-14186 Huddinge, Sweden Huddinge Univ Hosp, Karolinska Inst, Gene Therapy Ctr, S-14186 Huddinge, Sweden Huddinge Univ Hosp Huddinge Sweden S-14186 Ctr, S-14186 Huddinge, Sweden
Titolo Testata:
JOURNAL OF INTERNAL MEDICINE
fascicolo: 5, volume: 250, anno: 2001,
pagine: 373 - 381
SICI:
0954-6820(200111)250:5<373:EOIIOP>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; MODELS; VEGF;
Keywords:
coronary artery disease; gene therapy; plasmid; thoracotomy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Sylven, C Huddinge Univ Hosp, Karolinska Inst, Dept Cardiol, S-14186 Huddinge, Sweden Huddinge Univ Hosp Huddinge Sweden S-14186 86 Huddinge, Sweden
Citazione:
N. Sarkar et al., "Effects of intramyocardial injection of phVEGF-A(165) as sole therapy in patients with refractory coronary artery disease - 12-month follow-up: Angiogenic gene therapy", J INTERN M, 250(5), 2001, pp. 373-381

Abstract

Objective. To test the safety and bioactivity of phVEGF-A(165) after intramyocardial injection during 12-month follow-up. Design. Open-labelled study. Subjects. Inclusion criteria were angina pectoris, Canadian CardiovascularSociety (CCS) class III-IV, unamenable to further revascularization, ejection fraction (EF) >30%, perfusion defects extending over >10% of the anterolateral left ventricle wall detectable with adenosine single photon emission computerized tomography (SPECT) and at least one patent vessel visible bycoronary angiography. Seven of 39 patients referred for gene therapy were included. Intervention. Via a mini-thoracotomy under general anaesthesia, phVEGF-A(165) was injected directly into the myocardium at four sites in the anterolateral region of the left ventricle. Results. Operative procedures were uneventful. Perioperative release of myocardial markers and electrocardiogram (ECG) changes were detected in two patients. There were no perioperative deaths but one patient died 7 months postoperatively because of myocardial infarction. Plasma vascular endothelial growth factor (VEGF)-A levels increased two to threefold peaking 6 days postoperatively (P<0.004) and returning to baseline by day 30. A significantreduction in angina pectoris was reported. The CCS class improved from 3.3<plus/minus>0.2 to 1.9 +/-0.3 (P<0.01) and nitroglycerine intake decreased from 39<plus/minus>15 to 12 +/-5 tablets week(-1) (P<0.001) 2 months after gene transfer. Improvements remained after 12 months when nitroglycerine consumption approached zero. Improved myocardial function in the phVEGF-A(165) injection region was documented in all patients (P<0.016) by tissue velocity imaging (TVI). Reduced reversible ischaemia was detected by adenosine SPECT in four patients. Improved collateralization was detected in four patients with coronary angiography. Conclusion. Intramyocardial injection of phVEGF-A(165) is safe and may lead to improved myocardial perfusion and function with longstanding symptomatic relief in end-stage angina pectoris. Based on these results this therapeutic potential is being tested in a double-blind placebo controlled multicentre trial, EUROINJECT ONE.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:38:47