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Titolo:
In situ gelling and mucoadhesive polymer vehicles for controlled intranasal delivery of plasmid DNA
Autore:
Park, JS; Oh, YK; Yoon, H; Kim, JM; Kim, CK;
Indirizzi:
Seoul Natl Univ, Coll Pharm, Kwanak Ku, Seoul 151742, South Korea Seoul Natl Univ Seoul South Korea 151742 k Ku, Seoul 151742, South Korea Pochon CHA Univ, Coll Med, Dept Microbiol, Kyonggi Do, South Korea Pochon CHA Univ Kyonggi Do South Korea crobiol, Kyonggi Do, South Korea Pochon CHA Univ, Coll Med, Inst Med Res, Kyonggi Do, South Korea Pochon CHA Univ Kyonggi Do South Korea Med Res, Kyonggi Do, South Korea Pochon CHA Univ, Coll Med, Dept Anat, Kyonggi Do, South Korea Pochon CHA Univ Kyonggi Do South Korea pt Anat, Kyonggi Do, South Korea Hanyang Univ, Coll Med, Dept Microbiol, Seoul 133791, South Korea Hanyang Univ Seoul South Korea 133791 crobiol, Seoul 133791, South Korea Hanyang Univ, Coll Med, Inst Biomed Sci, Seoul 133791, South Korea HanyangUniv Seoul South Korea 133791 med Sci, Seoul 133791, South Korea
Titolo Testata:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
fascicolo: 1, volume: 59, anno: 2002,
pagine: 144 - 151
SICI:
0021-9304(200201)59:1<144:ISGAMP>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIQUID SUPPOSITORY; GENE-TRANSFER; RELEASE; PHARMACOKINETICS; BIOAVAILABILITY; ACETAMINOPHEN; COMPLEXES; SYSTEMS; VIVO; MICE;
Keywords:
in situ gelling; mucoadhesive polymers; nasal absorption; plasmid DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, CK Seoul Natl Univ, Coll Pharm, Kwanak Ku, San 56-1, Seoul 151742, South Korea Seoul Natl Univ San 56-1 Seoul South Korea 151742 42, South Korea
Citazione:
J.S. Park et al., "In situ gelling and mucoadhesive polymer vehicles for controlled intranasal delivery of plasmid DNA", J BIOMED MR, 59(1), 2002, pp. 144-151

Abstract

Nasal administration of plasmid DNA is emerging as a new route of deliveryfor therapeutic genes and DNA vaccines. To improve the intranasal absorption of plasmid DNA, we designed delivery systems composed of in situ gellingand mucoadhesive polymers. Poloxamers (Pol) were used to provide in situ gelling property. Polycarbophil (PC) or polyethylene oxide (PEO) was used asmucoadhesive polymers. The gelation temperatures of the formulations slightly decreased by the mucoadhesive polymers, but not by plasmid DNA. The in vitro release of plasmid DNA from the gels followed Fickian diffusion. The absorption of plasmid DNA varied with the contents and type of mucoadhesivepolymers. Of vehicles, Pol/PC 0.2% showed the highest absorption with an area under the curve value 11-fold higher than saline, the conventional vehicle. The nasal retention of plasmid DNA was highly prolonged by mucoadhesive polymers. At 3 h postdose, the nasal tissue levels of plasmid DNA given in Pol/PC and Pol/PEO 0.8% were 10- and 40-fold higher relative to saline. The histopathology of nasal tissues was not altered after repeated dosing over 2 weeks. The mRNA expression of plasmid DNA delivered by Pol or Pol/PEO 0.4% was observed in the nasal tissues. These results indicate that the nasal absorption of plasmid DNA can be effectively and safely enhanced by using in situ gelling and mucoadhesive polymer-based vehicles. (C) 2001 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/02/20 alle ore 03:10:29