Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Protein adsorption and human osteoblast-like cell attachment and growth onalkylthiol on gold self-assembled monolayers
Autore:
Scotchford, CA; Gilmore, CP; Cooper, E; Leggett, GJ; Downes, S;
Indirizzi:
Univ Nottingham, Sch Med, Sch Biomed Sci, Queens Med Ctr, Nottingham NG7 2UH, England Univ Nottingham Nottingham England NG7 2UH , Nottingham NG7 2UH, England Univ Nottingham, Sch Mech Mat Mfg Engn & Management, Nottingham NG7 2RD, England Univ Nottingham Nottingham England NG7 2RD , Nottingham NG7 2RD, England Univ Manchester, Inst Sci & Technol, Dept Chem, Manchester M60 1QD, Lancs,England Univ Manchester Manchester Lancs England M60 1QD r M60 1QD, Lancs,England Smith & Nephew Grp Res Ctr, York, N Yorkshire, England Smith & Nephew Grp Res Ctr York N Yorkshire England N Yorkshire, England
Titolo Testata:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
fascicolo: 1, volume: 59, anno: 2002,
pagine: 84 - 99
SICI:
0021-9304(200201)59:1<84:PAAHOC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ION MASS-SPECTROMETRY; HUMAN BONE; SURFACE-CHEMISTRY; FIBRONECTIN; FIBROBLASTS; ADHESION; POLYMERS; FILMS; RATES; PHOTOOXIDATION;
Keywords:
osteoblast; cell-surface interaction; alkylthiol self-assembled monolayers; surface chemistry; fibronectin; albumin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Scotchford, CA Univ Nottingham, Sch Med, Sch Biomed Sci, Queens Med Ctr, Nottingham NG7 2UH, England Univ Nottingham Nottingham England NG7 2UH 7 2UH, England
Citazione:
C.A. Scotchford et al., "Protein adsorption and human osteoblast-like cell attachment and growth onalkylthiol on gold self-assembled monolayers", J BIOMED MR, 59(1), 2002, pp. 84-99

Abstract

Protein adsorption and growth of primary human osteoblasts on self-assembled monolayers of alkylthiols on gold (SAMs) with carboxylic acid and hydroxyl and methyl termini were investigated. Single-component SAMs and SAMs patterned by photolithographic techniques were used. Cell growth on patterned SAMs demonstrated preferences for one pattern region in all combinations ofalkylthiols, with the hierarchical preference COOH > OH > CH3 Patterned SAMs and immunochemistry were used to investigate adsorption of fibronectin and albumin with respect to different alkylthiol termini. Fibronectin adsorption from both pure solution and serum containing cell culture medium (SDMEM) followed the sequence COOH > OH > CH3 Albumin adsorption from pure solution followed the sequence OH > COOH > CH3; from SDMEM the sequence was CH3 > OH > COOH. Cell attachment to SAMs with the above termini, after preadsorption with fibronectin, albumin, or mixtures of fibronectin and albumin, was measured. Attachment was maximal on COOH-terminated SAMs precoated with fibronectin. Attachment to COOH was significantly reduced only when fibronectin was omitted from the protein preadsorption solution. On OH and CH3 SAMsincreasing the proportion of albumin in the solution was sufficient to significantly reduce cell attachment. The distribution vinculin and the integrins alpha (5)beta (1) and alpha (nu)beta (3) indicated that focal contact formation by cells varied with alkylthiol termini in the following sequence:COOH > OH > CH. (C) 2001 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 23:42:54