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Titolo:
Bone morphogenetic protein 3b expressing reindeer antler
Autore:
Kapanen, A; Ryhanen, J; Birr, E; Vaananen, HK; Tuukkanen, J;
Indirizzi:
Univ Oulu, Dept Anat & Cell Biol, FIN-90014 Oulu, Finland Univ Oulu OuluFinland FIN-90014 at & Cell Biol, FIN-90014 Oulu, Finland Univ Oulu, Dept Surg, FIN-90014 Oulu, Finland Univ Oulu Oulu Finland FIN-90014 ulu, Dept Surg, FIN-90014 Oulu, Finland Univ Turku, Dept Anat, Inst Biomed, FIN-20520 Turku, Finland Univ Turku Turku Finland FIN-20520 Inst Biomed, FIN-20520 Turku, Finland
Titolo Testata:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
fascicolo: 1, volume: 59, anno: 2002,
pagine: 78 - 83
SICI:
0021-9304(200201)59:1<78:BMP3ER>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEER ANTLER; TISSUE; MATRIX; BMP-3B; DIFFERENTIATION; TRANSCRIPTS; COLLAGEN; MEMBER; FAMILY;
Keywords:
antler; reindeer; BMP-3b; heterotopic bone formation; pQCT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Kapanen, A Univ Oulu, Dept Anat & Cell Biol, FIN-90014 Oulu, Finland Univ Oulu Oulu Finland FIN-90014 iol, FIN-90014 Oulu, Finland
Citazione:
A. Kapanen et al., "Bone morphogenetic protein 3b expressing reindeer antler", J BIOMED MR, 59(1), 2002, pp. 78-83

Abstract

A cDNA sequence of bone morphogenetic protein 3b (BMP-3b) of reindeer antler was produced with degenerative homology primers in polymerase chain reaction (PCR). An in situ hybridization study of BMP-3b mRNA in I-month-old antler showed expression in most differentiated cells in the antler center. In addition, the bone-inductive capacity of the reindeer antler matrix was evaluated. Decalcified and powdered antler matrix of different stages of antler maturity was implanted in gelatin capsules under the rat dorsal muscle fascia for two implantation periods: 3 and 8 weeks. Allogenic matrix prepared from rat long bones was used as a positive control implant. Heterotopic ossification was evaluated histomorphometrically and densitometrically. Allogenic bone matrix induced rapid osteogenesis and mineral accumulation. Both endochondral and intramembranous ossification was evident, endochondral ossification being the dominant form. Mineral density in the induced ossiclewas 115 +/- 48 mg/cm(3) as early as at 3 weeks and 350 +/- 69 mg/cm(3) at 8 weeks. The proportional areas of von Kossa-stained mineral were 3.67 +/- 2.1% and 11.6 +/- 0.07%, respectively. The antler preparations induced mineralization, but significantly less than the allogenic bone matrix. At 8 weeks, mineral density was significantly lower in the cast antler preparation than in the allogenic implants. The morphology of the mineralized areas of the antler preparations showed no ossification. (C) 2001 John Wiley & Sons,Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 18:30:56