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Titolo:
Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels
Autore:
Bryant, SJ; Anseth, KS;
Indirizzi:
Univ Colorado, Dept Chem Engn, Boulder, CO 80309 USA Univ Colorado Boulder CO USA 80309 Dept Chem Engn, Boulder, CO 80309 USA Howard Hughes Med Inst, Chevy Chase, MD 20815 USA Howard Hughes Med Inst Chevy Chase MD USA 20815 Chevy Chase, MD 20815 USA
Titolo Testata:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
fascicolo: 1, volume: 59, anno: 2002,
pagine: 63 - 72
SICI:
0021-9304(200201)59:1<63:HPIEPB>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; ARTICULAR-CARTILAGE; INJECTABLE HYDROGELS; COLLAGEN; NETWORKS; SOLUTES; SURGERY; POLYMER; MATRIX; CELLS;
Keywords:
tissue engineering; cartilage; hydrogels; photopolymerization; poly(ethylene glycol);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Anseth, KS Univ Colorado, Dept Chem Engn, Boulder, CO 80309 USA Univ Colorado Boulder CO USA 80309 Engn, Boulder, CO 80309 USA
Citazione:
S.J. Bryant e K.S. Anseth, "Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels", J BIOMED MR, 59(1), 2002, pp. 63-72

Abstract

When using hydrogel scaffolds for cartilage tissue engineering, two gel properties are particularly important: the equilibrium water content (q, equilibrium swelling ratio) and the compressive modulus, K. In this work, chondrocytes were photoencapsulated in degrading and nondegrading poly(ethylene glycol)-based hydrogels to assess extracellular matrix (ECM) formation as afunction of these gel properties. In nondegrading gels, the glycosaminoglycan (GAG) content was not significantly different in gels when q was variedfrom 4.2 to 9.3 after 2 and 4 weeks in vitro. However, gels with a q of 9.3 allowed GAGs to diffuse throughout the gels homogenously, but a q less than or equal to 5.2 resulted in localization of GAGs pericellularly. Interestingly, in the moderately crosslinked gels with a K of 360 kPa, an increasein type II collagen synthesis was observed compared with gels with a higher (960 kPa) and lower (30 kPa) K after 4 weeks. With the incorporation of degradable linkages into the network, gel properties with an initially high K (350 kPa) and final high q (7.9) were obtained, which allowed for increased type II collagen synthesis coupled with a homogenous distribution of GAGs. Thus, a critical balance exists between gel swelling, mechanics, and degradation in forming a functional ECM. (C) 2001 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:23:36