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Titolo:
A hormone-dependent post-translationally regulated mutant for investigating type I cadherin function
Autore:
Giacomello, E; Morali, O; Vallin, J; Thiery, JP; Broders, F;
Indirizzi:
Inst Curie, Lab Morphogenese & Progress Tumorale, CNRS UMR 144, F-75248 Paris, France Inst Curie Paris France F-75248 ale, CNRS UMR 144, F-75248 Paris, France
Titolo Testata:
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
fascicolo: 7, volume: 45, anno: 2001,
pagine: 833 - 838
SICI:
0214-6282(200110)45:7<833:AHPRMF>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
STEROID-BINDING DOMAINS; XENOPUS-LAEVIS; CELL-ADHESION; BETA-CATENIN; C-CADHERIN; N-CADHERIN; EMBRYOS; MORPHOGENESIS; TRANSCRIPTION; PROTEINS;
Keywords:
type I cadherins; hormone-binding domain; Xenopus laevis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Giacomello, E Inst Curie, Lab Morphogenese & Progress Tumorale, CNRS UMR 144, 26 Rue Ulm, F-75248 Paris, France Inst Curie 26 Rue Ulm Paris France F-75248 48 Paris, France
Citazione:
E. Giacomello et al., "A hormone-dependent post-translationally regulated mutant for investigating type I cadherin function", INT J DEV B, 45(7), 2001, pp. 833-838

Abstract

Type I cadherins are Ca2+-dependent cell adhesion molecules. Their function in early Xenopus laevis development has been extensively studied in recent years, by injecting synthetic mRNAs encoding dominant negative mutants with deletions of the extracellular domain into embryos. However, studies at post-gastrula stages have been hampered by the inability to progress through post-gastrula development in embryos expressing these mutant proteins. This problem has been partly overcome by injecting into a few targeted blastomeres in stage 6 N.F. embryos, but only restricted studies are possible with this technique. Several studies have made use of the hormone-binding domain (HBD), which is activated by hormones. In this study, we used this method to analyze the activity of dominant negative cadherins. We generated a mutant E-cadherin (DeltaE-Cad, consisting of the cytoplasmic domain and transmembrane domain) fused to the hormone-binding domain of estradiol receptor (HBDER) and we validated this technique with functional analyses. The function of the mutant DeltaE-HBDER was strictly dependent on hormone induction. This conditional mutant had the same effects and exerted the same dominantnegative function as the corresponding constitutive mutant.

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Documento generato il 25/09/20 alle ore 21:51:48