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Titolo:
Esterase isozyme polymorphism, specific and nonspecific esterase, syngeniclines development and natural occurrence of a thermostable esterase in thetropical silkworm Bombyx mori L.
Autore:
Chattopadhyay, GK; Sengupta, AK; Verma, AK; Sen, SK; Saratchandra, B;
Indirizzi:
Cent Sericultural Res & Training Inst, Silkworm Breeding, Genet & Mol BiolLab, Murshidabad 742101, W Bengal, India Cent Sericultural Res & Training Inst Murshidabad W Bengal India 742101 a
Titolo Testata:
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
fascicolo: 12, volume: 31, anno: 2001,
pagine: 1191 - 1199
SICI:
0965-1748(20011101)31:12<1191:EIPSAN>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
BIOCHEMICAL PARAMETERS; MULBERRY SILKWORM;
Keywords:
CB5 (GP); digestive juice; haemolymph; esterase polymorphism; germload tolerant; syngenic line; heterogeneity; genepool; specific and nonspecific esterase; recurrent backcross line (RBL); congenic line (Con. L);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Chattopadhyay, GK Cent Sericultural Res & Training Inst, Silkworm Breeding, Genet & Mol BiolLab, Murshidabad 742101, W Bengal, India Cent Sericultural Res & Training Inst Murshidabad W Bengal India 742101
Citazione:
G.K. Chattopadhyay et al., "Esterase isozyme polymorphism, specific and nonspecific esterase, syngeniclines development and natural occurrence of a thermostable esterase in thetropical silkworm Bombyx mori L.", INSEC BIO M, 31(12), 2001, pp. 1191-1199

Abstract

Esterase isozyme polymorphism was documented for digestive juice and haemolymph of the tropical multivoltine silkworm, Bombyx mori L., breed CB5 (GP)and its syngenic lines (CB5LM(e)-1, CB5Lm-2 and CB5Lm-5) using alpha- and beta -naphthylacetate separately as nonspecific substrates (Ogita, Z., Kasai, T., 1965. Genetico-biochemical analysis of specific esterases in Musca domestica. Jpn. J. Genet. 40, 173-184). Polymorphism existed in the isozyme pattern of alpha -esterase with two or three bands in digestive juice and three to five bands in haemolymph. No polymorphism was observed in beta -esterase isozyme pattern having four bands in digestive juice and two bands inhaemolymph. During the course of esterase isozyme studies, the presence ofsome specific alpha -esterase bands (Est-1, 4 and 5) in haemolymph and beta -esterase bands (Est-1, 2 and 3) in digestive juice were observed. But both alpha- and beta -esterase bands Est-3 and 4 in digestive juice and Est-2and 3 in haemolymph were found to be nonspecific. Nonspecific beta -esterase band (Est-3) in haemolymph of CB5 (GP) and its syngenic lines withstood a temperature up to 80 +/-1 degreesC for 10 min. No thermostable band was observed in the isozyme zymogram of alpha -esterase in digestive juice and haemolymph or beta -esterase in digestive juice. Overall, this study discusses the presence of esterase heterogeneity in the CB5 (GP) genepool, syngenic lines development, occurrence of specific alpha- and beta -esterase bandsin digestive juice and haemolymph and thermostable beta -esterase band Est-3 in haemolymph in tropical silkworm Bombyx mori L. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 22:54:23