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Titolo:
Glutamate induced modulation of free Ca2+ in isolated inner hair cells of the guinea pig cochlea
Autore:
Li, XQ; Sun, JH; Yu, N; Sun, YR; Tan, ZL; Jiang, SC; Li, N; Zhou, CX;
Indirizzi:
Chinese Peoples Liberat Army Gen Hosp, Inst Otolaryngol, Beijing 100853, Peoples R China Chinese Peoples Liberat Army Gen Hosp Beijing Peoples R China 100853 ina
Titolo Testata:
HEARING RESEARCH
fascicolo: 1-2, volume: 161, anno: 2001,
pagine: 29 - 34
SICI:
0378-5955(200111)161:1-2<29:GIMOFC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMPA RECEPTOR SUBUNITS; RAT ORGAN; LOCALIZATION; SYNAPSES; CALCIUM; CORTI;
Keywords:
glutamate; inner hair cell; cochlea; intracellular free calcium; guinea pig;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Li, XQ Chinese Peoples Liberat Army Gen Hosp, Inst Otolaryngol, Beijing 100853, Peoples R China Chinese Peoples Liberat Army Gen Hosp Beijing Peoples R China 100853
Citazione:
X.Q. Li et al., "Glutamate induced modulation of free Ca2+ in isolated inner hair cells of the guinea pig cochlea", HEARING RES, 161(1-2), 2001, pp. 29-34

Abstract

To explore the possible involvement of glutamate (Glu) in modulation of inner hair cell (IHC) functions, the glutamate (Glu) induced changes in intracellular free Ca2+ ([Ca2+](i)) concentration in isolated IHCs and outer hair cells (OHCs) of the guinea pig cochlea were investigated with fluo-3. a fluorescent probe for intracellular Ca2+. Their unique flask shape identified the IHCs with a distinct neck and spherical base with a large spherical nucleus. Normal cell shapes could be maintained for about 2 h. Fluorescence of fluo-3 was distributed in the whole isolated IHC with brighter staining nuclei. Static [Ca2+](i) remained constant within the observation period inthe absence of Glu. In the presence of a low concentration of Glu (3.85 muM), there was an increase of [Ca2+](i) in IHCs, whereas no obvious [Ca2+](i) change was found in OHCs. The increase of the fluorescence in IHCs reached peak level at 180 s and then gradually reduced at 400 s after the administration of Glu. The increases of [Ca2+](i), were observed in nine of 10 IHCs. but one IHC did not show any change. For 10 of the observed OHCs, seven showed no [Ca2+](i) change, and three showed minor reduction of [Ca2+](i). The increase of the Glu concentration resulted in a corresponding change of[Ca2+](i) in the IHCs after three times administration of Glu. These results suggest that Glu acts on the IHCs presynaptic autoreceptor in a positivefeedback manner. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 14/07/20 alle ore 05:37:16