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Titolo:
A noninvasive reporter system to image adenoviral-mediated gene transfer to ovarian cancer xenografts
Autore:
Chaudhuri, TR; Rogers, BE; Buchsbaum, DJ; Mountz, JM; Zinn, KR;
Indirizzi:
Univ Alabama, Dept Radiol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 ept Radiol, Birmingham, AL 35294 USA Univ Alabama, Dept Radiat Oncol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 diat Oncol, Birmingham, AL 35294 USA
Titolo Testata:
GYNECOLOGIC ONCOLOGY
fascicolo: 2, volume: 83, anno: 2001,
pagine: 432 - 438
SICI:
0090-8258(200111)83:2<432:ANRSTI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR-BINDING PEPTIDES; SOMATOSTATIN RECEPTORS; THYMIDINE KINASE; THERAPY; VECTOR; CLONING; MURINE; TC-99M; MODEL;
Keywords:
imaging; gene therapy; ovarian cancer; 99m-Tc;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Zinn, KR Univ Alabama, Dept Radiol, BDB 11,1530 3rd Ave S, Birmingham, AL 35294 USA Univ Alabama BDB 11,1530 3rd Ave S Birmingham AL USA 35294 94 USA
Citazione:
T.R. Chaudhuri et al., "A noninvasive reporter system to image adenoviral-mediated gene transfer to ovarian cancer xenografts", GYNECOL ONC, 83(2), 2001, pp. 432-438

Abstract

Objective. Gene therapy trials for ovarian cancer would benefit from a noninvasive imaging modality to detect the location and extent of gene transfer. The human type 2 somatostatin receptor gene (hSSTr2) was evaluated as a reporter gene for imaging adenoviral (Ad) gene transfer to ovarian cancer. Methods. A replication-incompetent Ad vector encoding hSSTr2 (Ad-hSSTr2) was used to infect SKOV3.ip1 cells in vitro and tumors growing in nude mice. Gamma camera imaging detected uptake of 99m-Tc-P2045 (a somatostatin analogue) due to expressed hSSTr2. Results. Specific uptake of 99m-Te-P2045 was imaged in Ad-hSSTr2-infected cells in vitro. Noninvasive in vivo imaging detected gene transfer to intraperitoneal tumors. Uptake of 99m-TcP2045 (percentage dose per gram of tumor) averaged 2.2 and 0.18 for Ad-hSSTr2-injected mice and controls, respectively. Conclusion. This study reports the first noninvasive imaging method for imaging gene transfer to ovarian cancer. A human gene therapy trial is planned. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/08/20 alle ore 04:16:36