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Titolo:
Bax-induced apoptosis as a novel gene therapy approach for carcinoma of the cervix
Autore:
Huh, WK; Gomez-Navarro, J; Arafat, WO; Xiang, JL; Marameshwar, PJ; Alvarez, RD; Barnes, MN; Curiel, DT;
Indirizzi:
Univ Alabama, Gene Therapy Ctr, Birmingham, AL 35249 USA Univ Alabama Birmingham AL USA 35249 herapy Ctr, Birmingham, AL 35249 USA Univ Alabama, Div Human Gene Therapy, Dept Med, Birmingham, AL 35249 USA Univ Alabama Birmingham AL USA 35249 , Dept Med, Birmingham, AL 35249 USA Univ Alabama, Dept Pathol, Birmingham, AL 35249 USA Univ Alabama Birmingham AL USA 35249 ept Pathol, Birmingham, AL 35249 USA Univ Alabama, Dept Surg, Birmingham, AL 35249 USA Univ Alabama BirminghamAL USA 35249 Dept Surg, Birmingham, AL 35249 USA Univ Alabama, Dept Obstet & Gynecol, Birmingham, AL 35249 USA Univ Alabama Birmingham AL USA 35249 & Gynecol, Birmingham, AL 35249 USA
Titolo Testata:
GYNECOLOGIC ONCOLOGY
fascicolo: 2, volume: 83, anno: 2001,
pagine: 370 - 377
SICI:
0090-8258(200111)83:2<370:BAAANG>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOVIRUS-MEDIATED TRANSFER; CANCER-CELLS; OVARIAN-CANCER; VECTOR; PHOSPHATIDYLSERINE; INHIBITION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Huh, WK Div Gynecol Oncol, 619 St South,OHB 538, Birmingham, AL 35249 USA Div Gynecol Oncol 619 St South,OHB 538 Birmingham AL USA 35249 USA
Citazione:
W.K. Huh et al., "Bax-induced apoptosis as a novel gene therapy approach for carcinoma of the cervix", GYNECOL ONC, 83(2), 2001, pp. 370-377

Abstract

Objective. The transfer of tumor suppresser genes has been shown to revertthe malignant phenotype. In this regard, bax is a pro-apoptotic molecule that also functions as a tumor suppresser. The purpose of this study was to evaluate bax as a gene therapeutic in the context of cervical cancer. Methods. Efficiency of viral transduction in cervical cancer cell lines and primary cervical cancer cells was evaluated with an adenoviral vector encoding green fluorescent protein and luciferase, respectively. We generated a recombinant adenoviral vector that encodes the box gene under inducible conditions. To this end, expression of this pro-apoptotic gene was controlled by a CreLoxP system. Following infection with the recombinant bax adenovirus, the viability of cervical cancer cell lines and primary cervical cancer cells was evaluated using crystal violet staining and FACS analysis. Apoptotic cell death was monitored using annexin V staining. Results. High levels of viral infection were observed in all cervical cancer cell lines (> 85%) and primary cervical cancer cells. Significant cytotoxicity was seen in all cervical cancer cells lines and, more importantly, patient-derived primary cervical cancer cells. Moreover, bax-mediated cell death occurred via an apoptotic pathway. Conclusions. Our results indicate that a bax recombinant adenoviral vectorcauses cell death mediated via an apoptotic pathway in multiple cervical cancer cell lines and primary cervical cancer cells. These data suggest thatbox may be a candidate for human gene therapy in the setting of cervical carcinoma. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 16:02:39