Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Characterization of the gene EPAC2: Structure, chromosomal localization, tissue expression, and identification of the liver-specific isoform
Autore:
Ueno, H; Shibasaki, T; Iwanaga, T; Takahashi, K; Yokoyama, Y; Liu, LM; Yokoi, N; Ozaki, N; Matsukura, S; Yano, H; Seino, S;
Indirizzi:
Chiba Univ, Grad Sch Med, Dept Cellular & Mol Med, Chuo Ku, Chiba 2608670,Japan Chiba Univ Chiba Japan 2608670 r & Mol Med, Chuo Ku, Chiba 2608670,Japan Hokkaido Univ, Grad Sch Vet Med, Lab Anat, Sapporo, Hokkaido 0600818, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600818 oro, Hokkaido 0600818, Japan Chiba Univ, Grad Sch Med, Dept Mol Immunol, CREST,Chuo Ku, Chiba 2608670, Japan Chiba Univ Chiba Japan 2608670 unol, CREST,Chuo Ku, Chiba 2608670, Japan Okayama Univ, Sch Med, Dept Pediat, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 , Dept Pediat, Okayama 7008558, Japan Chiba Univ, Sch Med, Dept Med Genet, Chuo Ku, Chiba 2608670, Japan Chiba Univ Chiba Japan 2608670 Med Genet, Chuo Ku, Chiba 2608670, Japan Miyazaki Med Coll, Dept Internal Med 3, Miyazaki 8891692, Japan Miyazaki Med Coll Miyazaki Japan 8891692 Med 3, Miyazaki 8891692, Japan
Titolo Testata:
GENOMICS
fascicolo: 1-2, volume: 78, anno: 2001,
pagine: 91 - 98
SICI:
0888-7543(200111)78:1-2<91:COTGES>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; CAMP; RAP1; FAMILY; EXOCYTOSIS; CHANNEL; PATHWAY; BRAIN; RAS;
Keywords:
alternative splicing; cAMP; GEF; Rap1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Seino, S Chiba Univ, Grad Sch Med, Dept Cellular & Mol Med, Chuo Ku, 1-8-1Inohana,Chiba 2608670, Japan Chiba Univ 1-8-1 Inohana Chiba Japan 2608670hiba 2608670, Japan
Citazione:
H. Ueno et al., "Characterization of the gene EPAC2: Structure, chromosomal localization, tissue expression, and identification of the liver-specific isoform", GENOMICS, 78(1-2), 2001, pp. 91-98

Abstract

The liver-specific protein cAMP-GEFII (also known as Epac2) belongs to a family of cyclic adenosine monophosphate (cAMP) binding proteins having guanine nucleotide exchange factor (GEF) activity (the cAMP-GEF family). Here we clone the gene EPAC2, encoding cAMP-GEFII, from a human liver cDNA library. Human EPAC2 has at least 31 exons and is mapped to human chromosome 2q31. Analyses by primer extension, reverse transcriptase-polymerase chain reaction, and in situ hybridization revealed the presence of three transcription start sites of liver-specific Epac2: two major sites! located in exon 10 and a minor site in intron 9. The same translation start site is used in all three transcripts. Liver-specific cAMP-GEFII protein, which lacks the first cAMP-binding domain and the Dishevelled/Eg1-10/Pleckstrin domain, was detected at 79 kDa by immunoblot analysis, confirming the presence of the short form of cAMP-GEFII in the liver. Liver-specific cAMP-GEFII also has GEF activity toward Rap1. These results demonstrate the presence of liver-specific cAMP-GEFII Together with the previous finding that cAMP-GEFII is responsible for cAMP-dependent exocytosis in secretory cells, our study suggests that cAMP-GEFII may have a distinct role in liver.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 13:01:57