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Titolo:
Temperature-modulated array high-performance liquid chromatography
Autore:
Premstaller, A; Xiao, WH; Oberacher, H; OKeefe, M; Stern, D; Willis, T; Huber, CG; Oefner, PJ;
Indirizzi:
Stanford Genome Technol Ctr, Palo Alto, CA 94304 USA Stanford Genome Technol Ctr Palo Alto CA USA 94304 alo Alto, CA 94304 USA Leopold Franzens Univ, Inst Analyt Chem & Radiochem, A-6020 Innsbruck, Austria Leopold Franzens Univ Innsbruck Austria A-6020 A-6020 Innsbruck, Austria Affymetrix Inc, Santa Clara, CA 95051 USA Affymetrix Inc Santa Clara CA USA 95051 ix Inc, Santa Clara, CA 95051 USA
Titolo Testata:
GENOME RESEARCH
fascicolo: 11, volume: 11, anno: 2001,
pagine: 1944 - 1951
SICI:
1088-9051(200111)11:11<1944:TAHLC>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIPLEXED CAPILLARY-ELECTROPHORESIS; SINGLE-NUCLEOTIDE POLYMORPHISMS; DENATURING HPLC; MUTATIONS; DNA; GENES; BRCA1; DHPLC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Oefner, PJ Stanford Genome Technol Ctr, Palo Alto, CA 94304 USA Stanford Genome Technol Ctr Palo Alto CA USA 94304 A 94304 USA
Citazione:
A. Premstaller et al., "Temperature-modulated array high-performance liquid chromatography", GENOME RES, 11(11), 2001, pp. 1944-1951

Abstract

Using novel monolithic poly(styrene-divinylbenzene) capillary columns withail internal diameter of 0.2 mm, we demonstrate for the first time the feasibility of constructing high-performance liquid chromatography arrays for the detection of mutations by heteroduplex analysis under partially denaturing conditions. In one embodiment, such an array can be used to analyze onesample simultaneously at different temperatures to maximize the detection of mutations in DNA fragments containing multiple discrete melting domains. Alternatively, one may inject different samples onto columns kept at the same effective temperature. Further improvements in throughput can be obtained by means of laser-induced fluorescence detection and the differential labeling of samples with up to four different fluorophores. Major advantages of monolithic capillary high-performance liquid chromatographic arrays overtheir capillary electrophoretic analogs are the chemical inertness of the poly(styrene-divinylbenzene) stationary phase, the physical robustness of the Column bed due to its covalent linkage to the inner surface of the fusedsilica capillary, and the feasibility to modify the stationary phase thereby allowing the separation of compounds not only on the principle of size exclusion, but also adsorption, distribution, and ion exchange. Analyses times are on the order of a few Minutes and turnaround time is extremely shortas there is no need for the replenishment of the separation matrix betweenruns.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:36:46