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Titolo:
Co-transcriptional splicing of pre-messenger RNAs: considerations for the mechanism of alternative splicing
Autore:
Goldstrohm, AC; Greenleaf, AL; Garcia-Blanco, MA;
Indirizzi:
Duke Univ, Med Ctr, Dept Genet, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 , Med Ctr, Dept Genet, Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Microbiol, Durham, NC 27710 USA Duke Univ DurhamNC USA 27710 d Ctr, Dept Microbiol, Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Med Ctr, Dept Biochem, Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Med, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 iv, Med Ctr, Dept Med, Durham, NC 27710 USA
Titolo Testata:
GENE
fascicolo: 1-2, volume: 277, anno: 2001,
pagine: 31 - 47
SICI:
0378-1119(20011017)277:1-2<31:CSOPRC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR RECEPTOR-2; CARBOXY-TERMINAL DOMAIN; POLYMERASE-II ELONGATION; TRACT BINDING-PROTEIN; SITE SELECTION; IN-VITRO; FUNCTIONAL ASSOCIATION; BRANCHPOINT SEQUENCE; ACTIVATION DOMAINS; PREMESSENGER RNA;
Keywords:
pre-mRNA splicing; transcription; splice site-tethering; dynamic exon definition;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
137
Recensione:
Indirizzi per estratti:
Indirizzo: Garcia-Blanco, MA Duke Univ, Med Ctr, Dept Genet, POB 3053,424 CARL Bldg, Durham, NC 27710 USA Duke Univ POB 3053,424 CARL Bldg Durham NC USA 27710 USA
Citazione:
A.C. Goldstrohm et al., "Co-transcriptional splicing of pre-messenger RNAs: considerations for the mechanism of alternative splicing", GENE, 277(1-2), 2001, pp. 31-47

Abstract

Nascent transcripts are the true substrates for many splicing events in mammalian cells. In this review we discuss transcription, splicing, and alternative splicing in the context of co-transcriptional processing of pre-mRNA. The realization that splicing occurs co-transcriptionally requires two important considerations: First, the cis-acting elements in the splicing substrate are synthesized at different times in a 5' to 3' direction. This dynamic view of the substrate implies that in a 100 kb intron the 5' splice site will be synthesized as much as an hour before the 3' splice site. Second,the transcription machinery and the splicing machinery, which are both complex and very large, are working in close proximity to each other. It is therefore likely that these two macromolecular machines interact, and recent data supporting this notion is discussed. We propose a model for co-transcriptional pre-mRNA processing that incorporates the concepts of splice site-tethering and dynamic exon definition. Also, we present a dynamic view of the alternative splicing of FGF-R2 and suggest that this view could be generally applicable to many regulated splicing events. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/08/20 alle ore 16:56:09