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Titolo:
Identification of tropomyosin as a major allergen in the octopus Octopus vulgaris and elucidation of its IgE-binding epitopes
Autore:
Ishikawa, M; Suzuki, F; Ishida, M; Nagashima, Y; Shiomi, K;
Indirizzi:
Tokyo Univ Fisheries, Dept Food Sci & Technol, Tokyo 1088477, Japan Tokyo Univ Fisheries Tokyo Japan 1088477 & Technol, Tokyo 1088477, Japan
Titolo Testata:
FISHERIES SCIENCE
fascicolo: 5, volume: 67, anno: 2001,
pagine: 934 - 942
SICI:
0919-9268(200110)67:5<934:IOTAAM>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUSCLE PROTEIN TROPOMYOSIN; OYSTER CRASSOSTREA-GIGAS; SHRIMP ALLERGEN; PARVALBUMIN; CLONING; COD;
Keywords:
IgE-binding epitope; Octopus vulgaris; octopus; tropomyosin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Shiomi, K Tokyo Univ Fisheries, Dept Food Sci & Technol, Tokyo 1088477, Japan Tokyo Univ Fisheries Tokyo Japan 1088477 Tokyo 1088477, Japan
Citazione:
M. Ishikawa et al., "Identification of tropomyosin as a major allergen in the octopus Octopus vulgaris and elucidation of its IgE-binding epitopes", FISHERIES S, 67(5), 2001, pp. 934-942

Abstract

The major allergen (named Oct v 1) in the muscle of the octopus Octopus vulgaris was purified by gel filtration on Sephacryl S-300, anion-exchange fast protein liquid chromatography on Mono Q and reverse-phase high-pressure liquid chromatography on TSKgel Octadecyl-4PW. In addition to the molecularmass, amino acid composition and cross-reactivity with Tur c 1 (turban shell Turbo cornutus allergen), the determined partial amino acid sequence clearly demonstrated that Oct v 1 is tropomyosin, similar to the known molluscan and crustacean allergens. Using peptide fragments isolated from the lysylendopeptidase digest of Oct v 1, competitive enzyme-linked immunosorbant assay inhibition experiments showed that IgE-binding epitopes of Oct v 1 arecontained in two peptides (77-112 and 148-160) in the central region and one peptide (269-281) in the C-terminal region. In the peptide 77-112, the same sequence as the IgE-binding epitope proposed for Cra g 1 (oyster Crassostrea gigas allergen) is recognized at 92-105. Moreover, the peptide 148-160 partly overlaps with the IgE-binding epitopes suggested for Pen i 1 (shrimp Penaeus indicus allergen) and Pen a 1 (shrimp Penaeus aztecus allergen),and the peptide 269-281 with those for Tur c 1 and Pen a 1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 09:09:17