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Titolo:
Random amplified polymorphic DNA markers for three Japanese laminarian species
Autore:
Yotsukura, N; Kawai, T; Motomura, T; Ichimura, T;
Indirizzi:
Hokkaido Univ, Fac Sci, Inst Algol Res, Muroran, Hokkaido 0510003, Japan Hokkaido Univ Muroran Hokkaido Japan 0510003 ran, Hokkaido 0510003, Japan Hokkaido Fisheries Expt Stn, Yoichi, Hokkaido 0468555, Japan Hokkaido Fisheries Expt Stn Yoichi Hokkaido Japan 0468555 0468555, Japan
Titolo Testata:
FISHERIES SCIENCE
fascicolo: 5, volume: 67, anno: 2001,
pagine: 857 - 862
SICI:
0919-9268(200110)67:5<857:RAPDMF>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAPD MARKERS; PHAEOPHYTA; IDENTIFICATION; DISCRIMINATION; CULTIVARS; PRIMERS; FUCALES;
Keywords:
genetic marker; Japanese kelp; Laminaria; random amplified polymorphic DNA; species identification;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Yotsukura, N Hokkaido Univ, Fac Sci, Inst Algol Res, Muroran, Hokkaido 0510003, Japan Hokkaido Univ Muroran Hokkaido Japan 0510003 0510003, Japan
Citazione:
N. Yotsukura et al., "Random amplified polymorphic DNA markers for three Japanese laminarian species", FISHERIES S, 67(5), 2001, pp. 857-862

Abstract

Random amplified polymorphic DNA (RAPD) was studied to detect genetic markers three economically important Japanese laminarian species, Laminaria japonica-Areschoug, L. religiosa Miyabe and L. ochotensis Miyabe, which were sampled from their representative localities on the coasts from Tsugaru Straits to the Sea of Japan. DNA templates for RAPD were extracted from lamina using a DNA extraction kit and were purified with glassmilk. Reproducible RAPD markers for these three species were detected using three random primers from a total of 15 tested. In L. japonica and L. religiosa, these RAPD markers were confirmed to be useful for populations from other localities. The three species studied showed high intraspecific and interspecific band sharing index (BSI) values. Like annual typical L. religiosa from other localities, biennial individuals identified as putative L. religiosa from Atsutacould be discriminated from L. japonica but not from L. ochotensis by any of the RAPD genetic markers studied so far.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 11:54:00