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Titolo:
Altered cultured mesangial cell phenotypes from RF/J mice: A spontaneous immune complex mediated glomerulonephritis with progressive glomerulosclerosis
Autore:
Yashiro, M; Muso, E; Kamata, T; Oyama, A; Sasayama, S; Yoshida, H;
Indirizzi:
Kyoto Univ Hosp, Dept Internal Med, Div 3, Sakyo Ku, Kyoto 606, Japan Kyoto Univ Hosp Kyoto Japan 606 l Med, Div 3, Sakyo Ku, Kyoto 606, Japan Kyoto City Hosp, Div Nephrol, Kyoto, Japan Kyoto City Hosp Kyoto JapanKyoto City Hosp, Div Nephrol, Kyoto, Japan Fukui Med Univ, Clin Lab Med, Fukui, Japan Fukui Med Univ Fukui JapanFukui Med Univ, Clin Lab Med, Fukui, Japan
Titolo Testata:
EXPERIMENTAL NEPHROLOGY
fascicolo: 6, volume: 9, anno: 2001,
pagine: 420 - 427
SICI:
1018-7782(2001)9:6<420:ACMCPF>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; PDGF-RECEPTOR; EXTRACELLULAR-MATRIX; EPITHELIAL-CELLS; I COLLAGEN; EXPRESSION; KIDNEY; PROLIFERATION; SUBUNITS; ACID;
Keywords:
mesangial cells; platelet-derived growth factor; platelet-derived growth factor receptor; RF/J mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Muso, E Kyoto Univ Hosp, Dept Internal Med, Div 3, Sakyo Ku, 54 Kawaracho,Shogoin,Kyoto 606, Japan Kyoto Univ Hosp 54 Kawaracho,Shogoin Kyoto Japan 606 o 606, Japan
Citazione:
M. Yashiro et al., "Altered cultured mesangial cell phenotypes from RF/J mice: A spontaneous immune complex mediated glomerulonephritis with progressive glomerulosclerosis", EXP NEPHROL, 9(6), 2001, pp. 420-427

Abstract

Background/Aim: RF/J mice are a model of spontaneous immune complex mediated glomerulonephritis showing massive extracellular matrix accumulation andprogressive glomerulosclerosis. The aim of this study was to investigate whether there is an altered cultured mesangial cell (MC) phenotype in RF/J mice associated with these glomerular changes. Methods: The nature of cultured MCs from RF/J mice in the proliferative response to platelet-derived growth factor (PDGF) BB was compared with that of normal mice (BALB/c) by H-3-thymidine incorporation. The binding of PDGF-BB was examined with Scatchardanalysis, and the messenger RNAs (mRNAs) of PDGF beta -receptor, collagen I, collagen IV, and fibronectin were detected using Northern blot analysis in the MCs of each mouse. Results: The H-3-thymidine incorporation of MCs from RF/J mice showed significantly lower responses to PDGF-BB stimulations with concentrations ranging from 0.5 to 10.0 ng/ml in comparison with thoseof BALB/c mice which exhibited a proportional dose-dependent increase of the incorporation (p < 0.05 for 0.5 ng/ml PDGF-BB, p < 0.01 for 1.0-10.0 ng/ml). According to the Scatchard analysis, MCs from BALB/c mice showed aKD of 105 pM of PDGF-BB binding to its receptors, and the density of receptors was 5.82 fmol/10(5) cells. However, no binding PDGF-BB site on the surface of MCs from RF/J mice was noted. Northern blot analysis of MCs from RF/J mice indicated negative expression of detectable PDGF-beta receptor mRNA. As for matrix protein messages, MCs from RF/J mice did not express mRNA of type I collagen, but did express a higher amount of type IV collagen and fibronectin in comparison with MCs from normal BALB/c mice. Conclusions: An altered phenotype in MCs of RF/J mice was demonstrated, possibly contributing to the characteristic pathological glomerular changes. However, the precise association remains to be clarified. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 22:12:53