Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Electrophysiological analysis of suprachiasmatic nucleus projections to the ventrolateral preoptic area in the rat
Autore:
Sun, X; Whitefield, S; Rusak, B; Semba, K;
Indirizzi:
Dalhousie Univ, Dept Anat & Neurobiol, Halifax, NS B3H 4H7, Canada Dalhousie Univ Halifax NS Canada B3H 4H7 iol, Halifax, NS B3H 4H7, Canada Dalhousie Univ, Dept Physiol & Biophys, Halifax, NS B3H 4H7, Canada Dalhousie Univ Halifax NS Canada B3H 4H7 hys, Halifax, NS B3H 4H7, Canada Dalhousie Univ, Dept Psychol, Halifax, NS B3H 4J1, Canada Dalhousie Univ Halifax NS Canada B3H 4J1 hol, Halifax, NS B3H 4J1, Canada Dalhousie Univ, Dept Psychiat, Halifax, NS B3H 2E2, Canada Dalhousie UnivHalifax NS Canada B3H 2E2 iat, Halifax, NS B3H 2E2, Canada Dalhousie Univ, Dept Pharmacol, Halifax, NS B3H 4H7, Canada Dalhousie Univ Halifax NS Canada B3H 4H7 col, Halifax, NS B3H 4H7, Canada Dalhousie Univ, Cellular Microscopy & Digital Imaging Unit, Halifax, NS B3H 4H7, Canada Dalhousie Univ Halifax NS Canada B3H 4H7 nit, Halifax, NS B3H 4H7, Canada
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 8, volume: 14, anno: 2001,
pagine: 1257 - 1274
SICI:
0953-816X(200110)14:8<1257:EAOSNP>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYPOTHALAMIC PARAVENTRICULAR NUCLEUS; CIRCADIAN FIRING RHYTHMS; CENTRAL NERVOUS-SYSTEM; RAPID EYE-MOVEMENT; BASAL FOREBRAIN; SLEEP-DEPRIVATION; REM-SLEEP; NEURONS; LESIONS; WAKEFULNESS;
Keywords:
biocytin; GABA; galanin; glutamate; whole-cell patch-clamp recording;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Semba, K Dalhousie Univ, Dept Anat & Neurobiol, Tupper Med Bldg,Room 13MN,Halifax,NS B3H 4H7, Canada Dalhousie Univ Tupper Med Bldg,Room 13MN Halifax NS Canada B3H 4H7
Citazione:
X. Sun et al., "Electrophysiological analysis of suprachiasmatic nucleus projections to the ventrolateral preoptic area in the rat", EUR J NEURO, 14(8), 2001, pp. 1257-1274

Abstract

The circadian pacemaker housed in the suprachiasmatic nucleus (SCN) synchronizes daily sleep-wake cycles, presumably by modulating the sleep-wake regulatory system, including ventrolateral preoptic area (VLPO) neurons. We used whole-cell patch-clamp recording to study the projections from the SCN to the VLPO in horizontal slices of rat hypothalamus. Single-pulse stimulation of the SCN region elicited postsynaptic currents (PSCs) in 20 of 66 neurons (30%) recorded within the VLPO region as verified by intracellular biocytin labelling. At a holding potential of -60 mV, the evoked PSCs had an amplitude of 17.6 +/- 3.2 pA (SEM) and a latency of 6.3 +/- 0.5 ms (n = 10). There was a trend for simple excitatory postsynaptic currents (EPSCs) to beevoked in the VLPO cluster, simple inhibitory postsynaptic currents (IPSCs) in the extended VLPO, and a combination of EPSCs and IPSCs in both regions. IPSCs were blocked reversibly by bicuculline (10 muM, n = 11). In both the presence and absence of bicuculline, EPSCs had fast and slow components that were blocked by 6,7-dinitroquinoxaline-2,3-dione (DNQX; 10 muM; n = 7), and (+/-)3-(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP; 10 muM, n = 6), respectively. Reversal potentials for the evoked IPSCs and EPSCswere consistent with mediation via GABA(A) and ionotropic glutamate receptors, respectively. These results suggest that the SCN region provides both inhibitory and excitatory inputs to single VLPO neurons, which are mediated, respectively, by GABA(A) receptors and by both non-NMDA and NMDA glutamate receptors. These projections may play important roles in conveying circadian input to systems in the preoptic area that regulate sleep and waking.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 20:54:58