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Titolo:
Molecular cloning of a functional promoter of the human plakoglobin gene
Autore:
Potter, E; Braun, S; Lehmann, U; Brabant, G;
Indirizzi:
Hannover Med Sch, Dept Clin Endocrinol, D-30625 Hannover, Germany HannoverMed Sch Hannover Germany D-30625 nol, D-30625 Hannover, Germany Hannover Med Sch, Dept Nucl Med, D-30625 Hannover, Germany Hannover Med Sch Hannover Germany D-30625 Med, D-30625 Hannover, Germany Hannover Med Sch, Inst Pathol, D-30625 Hannover, Germany Hannover Med SchHannover Germany D-30625 hol, D-30625 Hannover, Germany
Titolo Testata:
EUROPEAN JOURNAL OF ENDOCRINOLOGY
fascicolo: 5, volume: 145, anno: 2001,
pagine: 625 - 633
SICI:
0804-4643(200111)145:5<625:MCOAFP>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTOR SNAIL; HUMAN THYROID-CARCINOMA; E-CADHERIN; BETA-CATENIN; GENOMIC ORGANIZATION; EMBRYONIC HEART; CPG ISLANDS; N-CADHERIN; CELL-LINES; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Brabant, G Hannover Med Sch, Dept Clin Endocrinol, Carl Neuberg Str 1, D-30625 Hannover, Germany Hannover Med Sch Carl Neuberg Str 1 Hannover Germany D-30625 y
Citazione:
E. Potter et al., "Molecular cloning of a functional promoter of the human plakoglobin gene", EUR J ENDOC, 145(5), 2001, pp. 625-633

Abstract

Objective: Plakoglobin (Pg) is the only cytoplasmic protein component common to both junctional complexes mediating cell-cell adhesion, adherens junctions and desmosomes. In these complexes Pg appears to act as a linker protein anchoring transmembrane proteins of the cadherin superfamily to the actin cytoskeleton and intermediate filament system respectively. Intercellular adhesion is frequently disturbed in skin diseases and in carcinomas, enabling tumour progression and metastasis. Whereas Pg expression is lost in some thyroid tumours and carcinoma cell lines, little information on Pg gene regulation is currently available owing to a lack of promoter studies. Design and methods: We have cloned and sequenced genomic DNA from a human library that resulted in 979 bp upstream of the published Pg cDNA. The transcriptional start was mapped by rapid amplification of cDNA ends. Methylation-specific PCR of bisulfite-modified cell fine DNA was applied to probe the methylation status of a promoter-associated CpG island. Reporter-gene constructs of various promoter fragments were transiently transfected in thyroid carcinoma cell lines and their activities were determined by luciferase measurements. Results and conclusions: A I kb DNA fragment harbouring a functional promoter of the human Pg gene was cloned and characterized. The sequence lacks acanonical TATA box, but contains putative CCAAT boxes as well as various putative binding sites for transcription factors, among them SP I and AP2, proximal to the transcriptional start. Considerable promoter activity was found in thyroid cell lines and deletion analysis indicated that a 300 bp region proximal to the 5'-untranslated region of the mRNA represents the minimal promoter of the human Pg gene. As cells lacking endogenous Pg expressionwere found to contain methylated CpG dinucleotides in a CpG island locatedaround the transcriptional start site, it is suggested that epigenetic mechanisms such as DNA methylation contribute to dysregulated Pg expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:16:32