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Titolo:
A role for coactivators and histone acetylation in estrogen receptor alpha-mediated transcription initiation
Autore:
Kim, MY; Hsiao, SJ; Kraus, WL;
Indirizzi:
Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 t Mol Biol & Genet, Ithaca, NY 14853 USA Cornell Univ, Grad Field Biochem Mol & Cell Biol, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 em Mol & Cell Biol, Ithaca, NY 14853 USA Cornell Univ, Weill Med Coll, Dept Pharmacol, New York, NY 10021 USA Cornell Univ New York NY USA 10021 Dept Pharmacol, New York, NY 10021 USA
Titolo Testata:
EMBO JOURNAL
fascicolo: 21, volume: 20, anno: 2001,
pagine: 6084 - 6094
SICI:
0261-4189(20011101)20:21<6084:ARFCAH>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-POLYMERASE-II; NUCLEAR-RECEPTOR; ACETYLTRANSFERASE ACTIVITY; CHROMATIN TEMPLATES; FUNCTIONAL STEPS; HORMONE-RECEPTOR; ONCOPROTEIN E1A; IN-VITRO; BINDING; P300;
Keywords:
chromatin; coactivator; estrogen receptor; histone acetylation; transcription;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Kraus, WL Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 & Genet, Ithaca, NY 14853 USA
Citazione:
M.Y. Kim et al., "A role for coactivators and histone acetylation in estrogen receptor alpha-mediated transcription initiation", EMBO J, 20(21), 2001, pp. 6084-6094

Abstract

Transcriptional regulation by estrogen receptor alpha (ER alpha) involves protein-protein interactions among the receptor, its associated coactivators and the RNA polymerase II transcriptional machinery. We have used an in vitro chromatin assembly and transcription system to examine the biochemistry of interactions among ER alpha, the SRC proteins and p300/CBP. Using polypeptides designed to block specific receptor- cofactor or cofactor-cofactorinteractions, we show that interactions among ER alpha, its coactivators and the RNA pol II machinery are all required for ER alpha- mediated transcription. Furthermore, we show that ER alpha -SRC-p300/CBP interactions are necessary and sufficient for the targeted acetylation of nucleosomal histones on estrogen-responsive promoters in the absence of transcription. The protein-protein interactions required for histone acetylation constitute a subset of the interactions required for transcriptional activation. Finally, we show that the major role of SRC-p300/CBP interactions is to enhance ER alpha- mediated transcription initiation, and they have little or no role in stimulating subsequent rounds of transcription. Together, our results indicate a specific role for the SRC and p300/CBP coactivators, as well as targeted histone acetylation, in ER alpha -mediated transcription.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 19:51:18