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Titolo:
Recruitment of protein kinase D to the trans-Golgi network via the first cysteine-rich domain
Autore:
Maeda, Y; Beznoussenko, GV; Van Lint, J; Mironov, AA; Malhotra, V;
Indirizzi:
Univ Calif San Diego, Div Biol, Dept Cell & Dev Biol, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 v Biol, La Jolla, CA 92093 USA Ist Ric Farmacol Mario Negri, Consorzio Mario Negri Sud, Dept Cell Biol & Oncol, I-66030 Santa Maria Imbaro, Chieti, Italy Ist Ric Farmacol Mario Negri Santa Maria Imbaro Chieti Italy I-66030 taly Katholieke Univ Leuven, Fac Geneeskunde, Afdeling Biochem, B-3000 Louvain,Belgium Katholieke Univ Leuven Louvain Belgium B-3000 em, B-3000 Louvain,Belgium
Titolo Testata:
EMBO JOURNAL
fascicolo: 21, volume: 20, anno: 2001,
pagine: 5982 - 5990
SICI:
0261-4189(20011101)20:21<5982:ROPKDT>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLECKSTRIN HOMOLOGY DOMAIN; C-MU; PHORBOL ESTER; D ACTIVATION; BREFELDIN-A; D PKD; BINDING; IDENTIFICATION; DIACYLGLYCEROL; MEMBRANES;
Keywords:
C1 domain; Golgi; PH domain; PKD; vesicular transport;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Malhotra, V Univ Calif San Diego, Div Biol, Dept Cell & Dev Biol, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 olla, CA 92093 USA
Citazione:
Y. Maeda et al., "Recruitment of protein kinase D to the trans-Golgi network via the first cysteine-rich domain", EMBO J, 20(21), 2001, pp. 5982-5990

Abstract

Protein kinase D (PKD) is a cytosolic protein, which upon binding to the trans-Golgi network (TGN) regulates the fission of transport carriers specifically destined to the cell surface. We have found that the first cysteine-rich domain (C1a), but not the second cysteine-rich domain (C1b), is sufficient for the binding of PKD to the TGN. Proline 155 in C1a is necessary forthe recruitment of intact PKD to the TGN. Whereas C1a is sufficient to target a reporter protein to the TGN, mutation of serines 744/748 to alanines in the activation loop of intact PKD inhibits its localization to the TGN. Moreover, anti-phospho-PKD antibody, which recognizes only the activated form of PKD, recognizes the TGN-bound PKD. Thus, activation of intact PKD is important for binding to the TGN.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 16:16:29