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Titolo:
Actin-dependent membrane association of the APC tumour suppressor in polarized mammalian epithelial cells
Autore:
Rosin-Arbesfeld, R; Ihrke, G; Bienz, M;
Indirizzi:
MRC, Mol Biol Lab, Cambridge CB2 2QH, England MRC Cambridge England CB2 2QH , Mol Biol Lab, Cambridge CB2 2QH, England Univ Cambridge, Wellcome Trust Ctr Mol Mech Dis, Cambridge CB2 2XY, England Univ Cambridge Cambridge England CB2 2XY Dis, Cambridge CB2 2XY, England Univ Cambridge, Dept Clin Biochem, Cambridge CB2 2XY, England Univ Cambridge Cambridge England CB2 2XY hem, Cambridge CB2 2XY, England
Titolo Testata:
EMBO JOURNAL
fascicolo: 21, volume: 20, anno: 2001,
pagine: 5929 - 5939
SICI:
0261-4189(20011101)20:21<5929:AMAOTA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYPOSIS-COLI PROTEIN; BETA-CATENIN; COLORECTAL-CANCER; DROSOPHILA; LOCALIZATION; ADHESION; MICE; TUMORIGENESIS; UVOMORULIN; EXPRESSION;
Keywords:
actin cytoskeleton; APC tumour suppressor; colorectal cancer cells; lateral plasma membrane; microtubules;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Bienz, M MRC, Mol Biol Lab, Hills Rd, Cambridge CB2 2QH, England MRC HillsRd Cambridge England CB2 2QH ambridge CB2 2QH, England
Citazione:
R. Rosin-Arbesfeld et al., "Actin-dependent membrane association of the APC tumour suppressor in polarized mammalian epithelial cells", EMBO J, 20(21), 2001, pp. 5929-5939

Abstract

Adenomatous polyposis coli (APC) is mutated in most colorectal cancers. APC downregulates nuclear beta -catenin, which is thought to be critical for its tumour suppressor function. However, APC may have additional and separate functions at the cell periphery. Here, we examine polarized MDCK and WIF-B hepatoma cells and find that APC is associated with their lateral plasmamembranes. This depends on the actin cytoskeleton but not on microtubules,and drug wash-out experiments suggest that APC is delivered continuously to the plasma membrane by a dynamic actin-dependent process. In polarized MDCK cells, APC also clusters at microtubule tips in their basal-most regions. Microtubule depolymerization causes APC to relocalize from these tips to the plasma membrane, indicating two distinct peripheral APC pools that are in equilibrium with each other in these cells. Truncations of APC such as those found in APC mutant cancer cells can neither associate with the plasmamembrane nor with microtubule tips. The ability of APC to reach the cell periphery may thus contribute to its tumour suppressor function in the intestinal epithelium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 07:00:54