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Titolo:
Muscle satellite cells are multipotential stem cells that exhibit myogenic, osteogenic, and adipogenic differentiation
Autore:
Asakura, A; Komaki, M; Rudnicki, MA;
Indirizzi:
Ottawa Hlth Res Inst, Program Mol Med, Ottawa, ON K1H 8L6, Canada Ottawa Hlth Res Inst Ottawa ON Canada K1H 8L6 Ottawa, ON K1H 8L6, Canada Univ Toronto, CIHR Periodontal Physiol, Fac Dent, Toronto, ON M5S 3E2, Canada Univ Toronto Toronto ON Canada M5S 3E2 Dent, Toronto, ON M5S 3E2, Canada
Titolo Testata:
DIFFERENTIATION
fascicolo: 4-5, volume: 68, anno: 2001,
pagine: 245 - 253
SICI:
0301-4681(200110)68:4-5<245:MSCAMS>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE MORPHOGENETIC PROTEIN-2; ADULT SKELETAL-MUSCLE; OSTEOBLAST DIFFERENTIATION; GENE-EXPRESSION; MYOD; MYF-5; ACTIVATION; MICE; TRANSPLANTATION; IDENTIFICATION;
Keywords:
satellite cell; stem cell; MyoD; myogenesis; osteogenesis; adipogenesis; differentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Rudnicki, MA Ottawa Hlth Res Inst, Program Mol Med, 501 Smyth Rd, Ottawa, ON K1H 8L6, Canada Ottawa Hlth Res Inst 501 Smyth Rd Ottawa ON Canada K1H 8L6 da
Citazione:
A. Asakura et al., "Muscle satellite cells are multipotential stem cells that exhibit myogenic, osteogenic, and adipogenic differentiation", DIFFERENTIA, 68(4-5), 2001, pp. 245-253

Abstract

Muscle satellite cells are believed to represent a committed stem cell population that is responsible for the postnatal growth and regeneration of skeletal muscle. However, the observation that cultured myoblasts differentiate into osteocytes or adipocytes following treatment with bone morphogenetic proteins (BMPs) or adipogenic inducers, respectively, suggests some degree of plasticity within the mesenchymal lineage. To further investigate thisphenomenon, we explore the osteogenic and adipogenic potential of satellite cells isolated from adult mice. Our experiments clearly demonstrate that satellite cell-derived primary myoblasts, expressing myogenic markers such as MyoD, Myf5, Pax7 and desmin, differentiated only into osteocytes or adipocytes following treatment with BMPs or adipogenic inducers, respectively. However, satellite cells on isolated muscle fibers cultured in Matrigel readily differentiated into myocytes as well as osteogenic and adipogenic lineages, whereas primary myoblasts did not. Satellite cell-derived primary myoblasts isolated from mice lacking the myogenic transcription factor MyoD (MyoD-/-) differentiate into myocytes poorly in vivo and in vitro (Megeney etal., Genes Dev. 1996; Sabourin et. al, J. Cell Biol., 1999). Therefore, wetested whether MyoD-/- primary myoblasts display increased plasticity relative to wild type cells. Unexpectedly, the osteogenic or adipogenic differentiation potential of MyoD-/- primary myoblasts did not increase compared to wild-type cells. Taken together, these results strongly suggest that muscle satellite cells possess multipotential mesenchymal stem cell activity and are capable of forming osteocytes and adipocytes as well as myocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 06:32:32