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Titolo:
A zymogel enhances the self-cleaning abilities of the skin of the pilot whale (Globicephala melas)
Autore:
Baum, C; Meyer, W; Roessner, D; Siebers, D; Fleischer, LG;
Indirizzi:
Hannover Sch Vet Med, Dept Histol & Embryol, Inst Anat, D-30173 Hannover, Germany Hannover Sch Vet Med Hannover Germany D-30173 D-30173 Hannover, Germany Alfred Wegener Inst Fdn Polar & Marine Res, D-27570 Bremerhaven, Germany Alfred Wegener Inst Fdn Polar & Marine Res Bremerhaven Germany D-27570 y Wyatt Technol Deutschland GMBH, D-57614 Woldert, Germany Wyatt Technol Deutschland GMBH Woldert Germany D-57614 Woldert, Germany Tech Univ Berlin, Inst Food Technol, D-13353 Berlin, Germany Tech Univ Berlin Berlin Germany D-13353 Technol, D-13353 Berlin, Germany Tech Univ Berlin, Ctr Biotechnol, D-13353 Berlin, Germany Tech Univ Berlin Berlin Germany D-13353 technol, D-13353 Berlin, Germany
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
fascicolo: 4, volume: 130, anno: 2001,
pagine: 835 - 847
SICI:
1095-6433(200111)130:4<835:AZETSA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL DIFFERENTIATION; DELPHINAPTERUS-LEUCAS; BOTTLENOSE DOLPHIN; TURSIOPS-TRUNCATUS; LIPOKERATINOCYTES; VISUALIZATION; GLYCOSIDASES; DESQUAMIN;
Keywords:
stratum corneum; enzymes; gel formation; epidermis; Globicephala melas; dolphins; skin protection; self-cleaning abilities;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Meyer, W Hannover Sch Vet Med, Dept Histol & Embryol, Inst Anat, Bischofsholer Damm15, D-30173 Hannover, Germany Hannover Sch Vet Med Bischofsholer Damm 15 Hannover Germany D-30173
Citazione:
C. Baum et al., "A zymogel enhances the self-cleaning abilities of the skin of the pilot whale (Globicephala melas)", COMP BIOC A, 130(4), 2001, pp. 835-847

Abstract

Enzyme activity in the stratum corneum of the pilot whale Globicephala melas was investigated employing colorimetric enzyme screening assays combinedwith NATIVE PAGE, size exclusion chromatography (SEC) and histochemical staining procedures. Applying different substrates, several enzymes were detected. The histochemical demonstration of some selected hydrolytic enzymes enriched in the stratum corneum showed high extracellular accumulation. As demonstrated by size exclusion chromatography, high molar mass aggregates were built up from a glycoprotein-rich 20-30-kD fraction. Using NATIVE PAGE experiments under non-reducing conditions, a selection of five degrading enzymes was recovered within the above-reported aggregates. Activity of extracellular aggregate-attached enzymes in the superficial layer of the stratum corneum exhibited no remarkable decrease potentially resulting from self-degradation. We thus conclude that due to their enclosure within the microenvironment of aggregates, a zymogel is formed and autolysis of the stratum corneum is reduced. With respect to the skin surface, the zymogel with hydrolytic activities covering major parts of it enhances the self-cleaning abilities of the skin of the pilot whale based on physical pre-requisites by hydrolyzing adhesive glycoconjugates of settling biofouling organisms considered as primary steps in fouling. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/01/20 alle ore 18:52:56