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Titolo:
Interleukin-12 prevents diaphragm muscle deterioration in a septic animal model
Autore:
Nakahata, E; Shindoh, Y; Takayama, T; Shindoh, C;
Indirizzi:
Tohoku Univ, Coll Med Sci, Dept Med Technol, Aoba Ku, Sendai, Miyagi 9808575, Japan Tohoku Univ Sendai Miyagi Japan 9808575 Ku, Sendai, Miyagi 9808575, Japan Sendai City Med Ctr, Dept Resp Med, Sendai, Miyagi, Japan Sendai City Med Ctr Sendai Miyagi Japan Resp Med, Sendai, Miyagi, Japan Tohoku Univ, Sch Med, Dept Surg 2, Sendai, Miyagi 980, Japan Tohoku Univ Sendai Miyagi Japan 980 ept Surg 2, Sendai, Miyagi 980, Japan
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
fascicolo: 4, volume: 130, anno: 2001,
pagine: 653 - 663
SICI:
1095-6433(200111)130:4<653:IPDMDI>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL STIMULATORY FACTOR; NATURAL-KILLER-CELLS; NITRIC-OXIDE; INTERFERON-GAMMA; SKELETAL-MUSCLE; SMOOTH-MUSCLE; T-CELLS; CYTOKINE; INDUCTION; SEPSIS;
Keywords:
endotoxin; free radicals; superoxide; nitric oxide; NADPH diaphorase; ATPase; cytokine; skeletal muscle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Shindoh, C Tohoku Univ, Coll Med Sci, Dept Med Technol, Aoba Ku, 2-1 Seiryo Machi, Sendai, Miyagi 9808575, Japan Tohoku Univ 2-1 Seiryo Machi Sendai Miyagi Japan 9808575 Japan
Citazione:
E. Nakahata et al., "Interleukin-12 prevents diaphragm muscle deterioration in a septic animal model", COMP BIOC A, 130(4), 2001, pp. 653-663

Abstract

The effects of an intravenous injection of Interleukin-12 (IL-12) after endotoxin administration and without endotoxin administration on diaphragm muscle were studied using Wistar rats. Three treatment groups, namely a control (Saline + endotoxin) group, an IL-12 + endotoxin group and an IL-12 onlygroup were studied. E. coli endotoxin (30 mg/kg) was injected intraperitoneally 5 min after Saline or IL-12 (0.25 mug) injection. In the control group, the force-frequency curves, twitch tension (TT) and slope during contraction time (TT/CT) were significantly lower at 4 h than those at 0 h due to endotoxin (P < 0.001, P < 0.01 and P < 0.01, respectively), and NO production was increased at 4 h as shown by NADPH diaphorase staining. In the IL-12+ endotoxin group, the decrement of the force-frequency curves, TT and TT/CT induced by endotoxin at 4 h were significantly prevented compared with those of the control group (P < 0.001, P < 0.05 and P < 0.05, respectively),and NO production was blocked at 4 h. In the IL-12 only group, the force-frequency curves were decreased in the range of high frequency and IL-12 resulted in NO production. Furthermore, the positive muscle fibers detected byNADPH diaphorase staining were classified as type I and IIa muscle fibers by ATPase staining in the control and IL-12 only groups. It is concluded that IL-12 prevents the deterioration of diaphragm muscle contraction inducedby endotoxin by reducing NO production in type I and IIa muscle fibers. These results suggest that IL-12 and endotoxin may interfere with each other. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:49:50