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Titolo:
Profiling of differentially expressed cancer-related genes in esophageal squamous cell carcinoma (ESCC) using human cancer cDNA arrays: Overexpression of oncogene MET correlates with tumor differentiation in ESCC
Autore:
Hu, YC; Lam, KY; Law, S; Wong, J; Srivastava, G;
Indirizzi:
Univ Hong Kong, Fac Med, Dept Pathol, Hong Kong, Hong Kong, Peoples R China Univ Hong Kong Hong Kong Hong Kong Peoples R China Kong, Peoples R China Univ Hong Kong, Fac Med, Dept Surg, Hong Kong, Hong Kong, Peoples R China Univ Hong Kong Hong Kong Hong Kong Peoples R China Kong, Peoples R China
Titolo Testata:
CLINICAL CANCER RESEARCH
fascicolo: 11, volume: 7, anno: 2001,
pagine: 3519 - 3525
SICI:
1078-0432(200111)7:11<3519:PODECG>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATOCYTE GROWTH-FACTOR; TYROSINE KINASE RECEPTOR; HUMAN BREAST-CARCINOMA; C-MET; MET/HGF RECEPTOR; CYCLIN D1; AMPLIFICATION; LINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Srivastava, G Univ Hong Kong, Queen Mary Hosp, Dept Pathol, Univ Pathol Bldg,Pok Fu Lam Rd, Hong Kong, Hong Kong, Peoples R China Univ Hong Kong UnivPathol Bldg,Pok Fu Lam Rd Hong Kong Hong Kong Peoples R China
Citazione:
Y.C. Hu et al., "Profiling of differentially expressed cancer-related genes in esophageal squamous cell carcinoma (ESCC) using human cancer cDNA arrays: Overexpression of oncogene MET correlates with tumor differentiation in ESCC", CLIN CANC R, 7(11), 2001, pp. 3519-3525

Abstract

Purpose: To examine the global gene expression of cancer-related genes in esophageal squamous cell carcinoma (ESCC) through the use of Atlas Human Cancer Array membranes printed with 588 well-characterized human genes involved in cancer and tumor biology. Experimental Design: Two human ESCC cell lines (HKESC-1 and HKESC-2) and one morphologically normal esophageal epithelium tissue specimen from the patient of which the RKESC-2 was derived were screened in parallel using cDNAexpression arrays. The array results were additionally validated using semiquantitative PCR. The overexpression of oncogene MET was studied more extensively for its protein expression by immunohistochemistry in the two ESCC cell lines and their corresponding primary tissues and 61 primary ESCC resected specimens. Sixteen of these 61 ESCC cases also had available the corresponding morphologically normal esophageal epithelium tissues and were alsoanalyzed for MET expression. The clinicopathological features associated with overexpression of the MET gene were also correlated. Results: The results of cDNA arrays showed that 13 cancer-related genes were up-regulated greater than or equal to2-fold (CDC25B, cyclin D1, PCNA, MET, Jagged 2, Integrin alpha3, Integrin alpha6, Integrin beta4, Caveolin-2, Caveolin-1, MMP13, MMP14, and BIGH3) and 5 genes were down-regulated greater than or equal to2-fold (CK4, Bad, IGFBP2, CSPCP, and IL-1RA) in both ESCCcell lines at the mRNA level. Semiquantitative RT-PCR analysis of 9 of these differentially expressed genes, including the MET gene, gave results consistent with cDNA array findings. The immunostaining results of the expression of MET gene showed that MET was overexpressed in both ESCC cell lines and their corresponding primary tumors at the protein level, validating the cDNA arrays findings. The results of the clinical specimens showed that theMET gene was overexpressed in ESCC compared with normal esophageal epithelium in 56 of 61 cases (92%). Moreover, the overexpression of MET protein was more often seen in well/moderately differentiated than in poorly differentiated ESCC. Conclusions: Multiple cancer-related genes are differentially expressed inESCC, the oncogene MET is overexpressed in ESCC compared with normal esophageal epithelium, and its protein overexpression correlates with tumor differentiation in ESCC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 01:30:37