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Titolo:
In vitro induction and in vivo expression of bcl-2 in the hNT neurons
Autore:
Daadi, MM; Saporta, S; Willing, AE; Zigova, T; McGrogan, MP; Sanberg, PR;
Indirizzi:
Layton Biosci Inc, Sunnyvale, CA 94086 USA Layton Biosci Inc Sunnyvale CAUSA 94086 sci Inc, Sunnyvale, CA 94086 USA Univ S Florida, Coll Med, Dept Neurosurg, Tampa, FL USA Univ S Florida Tampa FL USA ida, Coll Med, Dept Neurosurg, Tampa, FL USA Univ S Florida, Coll Med, Ctr Aging & Brain Repair, Tampa, FL USA Univ S Florida Tampa FL USA Med, Ctr Aging & Brain Repair, Tampa, FL USA Univ S Florida, Coll Med, Dept Anat, Tampa, FL USA Univ S Florida Tampa FL USA Florida, Coll Med, Dept Anat, Tampa, FL USA Univ Florida, Coll Med, Program Neurosci, Tampa, FL USA Univ Florida Tampa FL USA ida, Coll Med, Program Neurosci, Tampa, FL USA Univ Florida, Coll Med, Dept Psychiat Pharmacol & Psychol, Tampa, FL USA Univ Florida Tampa FL USA pt Psychiat Pharmacol & Psychol, Tampa, FL USA
Titolo Testata:
BRAIN RESEARCH BULLETIN
fascicolo: 2, volume: 56, anno: 2001,
pagine: 147 - 152
SICI:
0361-9230(20010915)56:2<147:IVIAIV>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
EMBRYONAL CARCINOMA-CELLS; POSTMITOTIC HUMAN NEURONS; NERVOUS-SYSTEM; CHROMOSOMAL BREAKPOINT; NEURAL TRANSPLANTATION; RETINOIC ACID; ISCHEMIC RATS; NTERA-2 CELLS; BRAIN INJURY; CDNA CLONES;
Keywords:
NT2 cell line; neural precursors; hNT neurons; retinoic acid; bcl-2 expression; differentiation; neuronal lineage; neural transplantation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Daadi, MM Layton Biosci Inc, 709 E Evelyn Ave, Sunnyvale, CA 94086 USA Layton Biosci Inc 709 E Evelyn Ave Sunnyvale CA USA 94086 86 USA
Citazione:
M.M. Daadi et al., "In vitro induction and in vivo expression of bcl-2 in the hNT neurons", BRAIN RES B, 56(2), 2001, pp. 147-152

Abstract

Bcl-2 encodes membrane-associated proteins that suppress programmed cell death in cells of various origins. Compelling evidence suggests that bcl-2 is also involved in neuronal differentiation and axonal regeneration. The human Neuro-Teratocarcinoma (hNT) neurons constitute a terminally differentiated human neuronal cell line that is derived from the Ntera-2/clone D1 (NT2) precursors upon retinoic acid (RA) treatment. After transplantation into the central nervous system (CNS), the hNT neurons survive, engraft, maintain their neuronal identity, and extend long neurite outgrowth. We were particularly interested in the intracellular determinants that confer these post-transplant characteristics to the hNT neurons. Thus, we asked whether the hNT neurons express bcl-2 after transplantation into the rat striatum and if RA induction of the neuronal lineage is mediated by bcl-2. The grafted hNT neurons were first identified using three different antibodies that recognize human-specific epitopes, anti-hMit, anti-hNuc, and NuMA. After a 1-month post-transplant survival time, NuMA immunostaining revealed that 12% of the hNT neurons survived the transplantation. These neurons extended long neuritic processes within the striatum, as demonstrated using the human-specific antibody against the midsize neurofilament subunit HO14. Importantly, we found that 85% of the implanted hNT neurons expressed bcl-2 and that thein vitro induction of the neuronal lineage from the NT2 precursors with RAresulted in an upregulation of bcl-2 expression. Together, these data suggest that the differentiation of the hNT neurons to a neuronal lineage couldbe mediated at least partially by bcl-2. (C) 2001 Elsevier Science Inc.

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Documento generato il 23/11/20 alle ore 21:48:08