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Titolo:
Human macrophage metalloelastase (MMP-12) expression is induced in chondrocytes during fetal development and malignant transformation
Autore:
Kerkela, E; Bohling, T; Herva, R; Uria, JA; Saarialho-Kere, U;
Indirizzi:
Univ Helsinki, Cent Hosp, Dept Dermatol, Helsinki 00250, Finland Univ Helsinki Helsinki Finland 00250 t Dermatol, Helsinki 00250, Finland Univ Helsinki, Cent Hosp, Dept Pathol, Helsinki 00250, Finland Univ Helsinki Helsinki Finland 00250 ept Pathol, Helsinki 00250, Finland Univ Oulu, Dept Pathol, Oulu, Finland Univ Oulu Oulu FinlandUniv Oulu, Dept Pathol, Oulu, Finland Univ Oviedo, Inst Oncol, Dept Bioquim & Biol Mol, Oviedo, Spain Univ Oviedo Oviedo Spain Oncol, Dept Bioquim & Biol Mol, Oviedo, Spain
Titolo Testata:
BONE
fascicolo: 5, volume: 29, anno: 2001,
pagine: 487 - 493
SICI:
8756-3282(200111)29:5<487:HMM(EI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; MATRIX METALLOPROTEINASES; COLLAGENASE-3 MMP-13; HYPERTROPHIC CHONDROCYTES; TISSUE INHIBITOR; WOUND REPAIR; CELL-LINE; CHONDROSARCOMA; ANGIOSTATIN; GROWTH;
Keywords:
bone; cartilage; chondrosarcoma; collagenase-3; embryogenesis; metalloproteinase (MMP);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Saarialho-Kere, U Univ Helsinki, Cent Hosp, Dept Dermatol, Meilahdentie 2,Helsinki 00250, Finland Univ Helsinki Meilahdentie 2 Helsinki Finland 00250 and
Citazione:
E. Kerkela et al., "Human macrophage metalloelastase (MMP-12) expression is induced in chondrocytes during fetal development and malignant transformation", BONE, 29(5), 2001, pp. 487-493

Abstract

Fetal development and tumor progression both require a complex system of extracellular matrix (ECM) synthesis and breakdown, which is mediated by, for instance, the matrix metalloproteinases (MMPs). Human metalloelastase (MMP-12) is an MMP, the expression of which has so far been documented in macrophages associated with atherosclerosis, wound repair, and certain cancers. In this study we first examined the expression of MMP-12 during human fetal development. By in situ hybridization MMP-12 transcripts were detected inchondrocytes of hypertrophic cartilage in vertebrae of the spinal column, in ribs, and in extremities undergoing ossification, beginning at the gestational age of 8 weeks. Also, periosteal cells expressed MMP-12 at 11 weeks. No expression of MMP-12 mRNA could be noted in other fetal tissues, including the skin, lungs, intestine, kidney, and liver. Expression of MMP-12 mRNA could not be detected in adult normal cartilage or osteosarcomas, but in chondrosarcomas both macrophages (8 of 19 samples) (identified by CD68 immunostaining) and chondrosarcoma cells (8 of 19) were positive. MMP-12 was also demonstrated in the tumors by western blotting and it was expressed in the same regions as MMP-13 mRNA. By immunostaining, MMP-12 mRNA colocalized with the protein in both fetal and chondrosarcoma specimens. Unlike basic fibroblast growth factor (bFGF) and transforming growth factor-beta (TGF-beta), tumor necrosis factor-alpha (TNF-alpha) induced MMP-12 mRNA production in chondrosarcoma-derived HTB-94 cells. Our results suggest that MMP-12 plays an important role in ECM remodeling during fetal bone development and isinduced when chondrocytes undergo malignant transformation. (C) 2001 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 09:08:47