Catalogo Articoli (Spogli Riviste)

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Titolo:
Spatial and temporal gene expression for fibroblast growth factor type I receptor (FGFR1) during fracture healing in the rat
Autore:
Nakajima, A; Nakajima, F; Shimizu, S; Ogasawara, A; Wanaka, A; Moriya, H; Einhorn, TA; Yamazaki, M;
Indirizzi:
Chiba Univ, Sch Med, Dept Orthopaed Surg, Chuo Ku, Chiba 2608677, Japan Chiba Univ Chiba Japan 2608677 opaed Surg, Chuo Ku, Chiba 2608677, Japan Fukushima Med Univ, Sch Med, Inst Biomed Sci, Dept Cell Sci, Fukushima, Japan Fukushima Med Univ Fukushima Japan Sci, Dept Cell Sci, Fukushima, Japan Boston Univ, Sch Med, Dept Orthopaed Surg, Boston, MA 02215 USA Boston Univ Boston MA USA 02215 Dept Orthopaed Surg, Boston, MA 02215 USA
Titolo Testata:
BONE
fascicolo: 5, volume: 29, anno: 2001,
pagine: 458 - 466
SICI:
8756-3282(200111)29:5<458:SATGEF>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
OSTEOCLAST FUNCTION; BONE-FORMATION; MESSENGER-RNA; DIFFERENTIATION; LOCALIZATION; MUTATIONS; PROTEIN-4; DISTINCT; KINASE; DOMAIN;
Keywords:
fibroblast growth factor receptor 1 (FGFR1); basic FGF (bFGF); gene expression; in situ hybridization; RNAse protection assay; fracture healing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Yamazaki, M Chiba Univ, Sch Med, Dept Orthopaed Surg, Chuo Ku, 1-8-1 Inohana, Chiba 2608677, Japan Chiba Univ 1-8-1 Inohana Chiba Japan 2608677 a 2608677, Japan
Citazione:
A. Nakajima et al., "Spatial and temporal gene expression for fibroblast growth factor type I receptor (FGFR1) during fracture healing in the rat", BONE, 29(5), 2001, pp. 458-466

Abstract

Recent experiments have shown that exogenous basic fibroblast growth factor (bFGF) enlarges fracture callus and accelerates the healing of osteotomized long bones. The actions of bFGF are mediated by four different transmembrane receptors (FGFR1-4). Among them, FGFR1 has a high affinity for bFGF, and gain-of-function mutations of the FGFR1 gene cause craniosynostosis in humans. Gene expression for FGFRI has been analyzed in embryogenesis-1 however, in skeletal repair, detailed expression of FGFRI has not been fully established. In the present study, a rat model of closed femoral fracture healing was used to quantify mRNA encoding the FGFRI and to characterize cells expressing FGFRI by in situ hybridization. Gene expression for FGFRI was rapidly upregulated after fracture; its mRNA level on day 1 was 3.4-fold higher than that of unfractured femora. At this stage, a moderate signal for FGFR1 was detected in periosteal osteoprogenitor cells, inflammatory cells near fracture sites, and cells among muscle layers. FGFR1 MRNA reached peak expression when callus remodeling actively progressed (6.8-fold on day 14), and remained elevated even in the later stages of healing (6.3-fold on day 28). During the intermediate stage of fracture healing, a strong signal forFGFR1 was diffusely distributed in mature osteoblasts in the hard callus, and mature osteoclasts also expressed a weak signal for FGFRI. These results suggest that FGF/FGFR1 signaling has multifunctional roles during fracture healing and may regulate both osteoblasts and osteoclasts, contributing to bone formation and callus remodeling. (C) 2001 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 10:27:42